Competitive Incorporation of Homologous Gene Segments of Influenza A Virus into Virions
Competitive Incorporation of Homologous Gene Segments of Influenza A Virus into Virions
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DOI：
10.1128/jvi.01204-12
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发表时间：
2012-09-01
影响因子：
5.4
通讯作者：
Kawaoka, Yoshihiro
中科院分区：
文献类型：
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作者：
Inagaki, Arisa;Goto, Hideo;Kawaoka, Yoshihiro
通过使用来自相同病毒RNA （vRNA）片段的两个报告蛋白编码病毒样RNA，我们评估了它们在单个子代病毒粒子中的整合效率。在两种报告基因均阳性的细胞中产生的重组病毒形成的大多数斑块只表达一种或另一种蛋白质。这些结果表明，编码不同报告蛋白的两种病毒样rna相互竞争以整合到病毒粒子中，并且单个流感病毒粒子整合同源vRNA片段的单个拷贝，而不是多个拷贝。
By using two reporter protein-encoding virus-like RNAs derived from identical viral RNA (vRNA) segments, we assessed their incorporation efficiency into single progeny virions. Most plaques formed by the recombinant viruses that were generated in cells positive for both reporter genes expressed only one or the other protein. These results suggest that two virus-like RNAs encoding different reporter proteins compete for incorporation into virions, and individual influenza virions incorporate single, but not multiple, copies of homologous vRNA segments.