Temporal and spatial action of Tolloid (Mini fin) and Chordin to pattern tail tissues
Temporal and spatial action of Tolloid (Mini fin) and Chordin to pattern tail tissues
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DOI：
10.1016/j.ydbio.2006.01.029
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发表时间：
2006-05-01
影响因子：
2.7
通讯作者：
Mullins, MC
中科院分区：
文献类型：
--
作者：
Connors, SA;Tucker, JA;Mullins, MC
在脊椎动物中，骨形态发生蛋白(BMP)信号通路沿着胚轴排列所有腹侧细胞的命运。BMP活性受金属蛋白酶Tolloid的正向调节，该酶可消除BMP拮抗剂Chordin的活性。斑马鱼的Tolloid突变体Mini Fin(MFN)表现出腹部尾巴组织的特异性丢失。在这里，我们研究了Tolloid(MFN)在尾部背腹模式中的空间和时间需求。通过嵌合分析，我们发现Tolloid(MFN)在原肠胚或其衍生物的腹面大多数植物细胞中发挥非自主的细胞功能。我们在可诱导的HSP70启动子的控制下产生了Tolloid转基因，并证明了Tolloid(MFN)是原肠形成完成时首先需要的。虽然Tolloid在原肠发育期间以及尾芽的背侧和腹侧表达，但我们的结果表明，Tolloid(MFN)在腹侧尾芽中特异地作用于从原肠形成完成到体细胞发生早期的4h时间段，以调节BMP信号。通过过表达HSP70-Tolloid转基因对Chordin活性的时间要求的研究表明，在原肠期间和之后的原肠形成过程中和之后都需要Chordin来正确地形成尾部，而TLD只在原肠发育后阶段需要。我们假设，原肠胚在尾部构型中的作用是产生适当大小的细胞结构域进入腹侧和背侧尾芽，而原肠胚后的原肠胚胶凝素活性模式是尾芽的衍生物。因此，通过分别通过Chordin和Tolloid的负和正作用对BMP信号水平进行精细调制，形成了尾部组织的模式。(C)2006 Elsevier Inc.保留所有权利。
In vertebrates, a bone morphogenetic protein (BMP) signaling pathway patterns all ventral cell fates along the embryonic axis. BMP activity is positively regulated by Tolloid, a metalloprotease, that can eliminate the activity of the BMP antagonist Chordin. A tolloid mutant in zebrafish, mini fin (mfn), exhibits a specific loss of ventral tail tissues. Here, we investigate the spatial and temporal requirements for Tolloid (Mfn) in dorsoventral patterning of the tail. Through chimeric analyses, we found that Tolloid (Mfn) functions cell non-autonomously in the ventral-most vegetal cells of the gastrula or their derivatives. We generated a tolloid transgene under the control of the inducible hsp70 promoter and demonstrate that tolloid (mfn) is first required at the completion of gastrulation. Although tolloid is expressed during gastrulation and dorsally and ventrally within the tail bud, our results indicate that Tolloid (Mfn) acts specifically in the ventral tail bud during a similar to 4 h period extending from the completion of gastrulation to early somitogenesis stages to regulate BMP signaling.Examination of the temporal requirements of Chordin activity by overexpression of the hsp70-tolloid transgene indicates that Chordin is required both during and after gastrulation for proper patterning of the tail, contrasting Tld's requirement only during post-gastrula stages. We hypothesize that the gastrula role of Chordin in tail patterning is to generate the proper size domains of cells to enter the ventral and dorsal tail bud, whereas post-gastrula Chordin activity patterns the derivatives of the tail bud. Thus, fine modulation of BMP signaling levels through the negative and positive actions of Chordin and Tolloid, respectively, patterns tail tissues. (c) 2006 Elsevier Inc. All rights reserved.