A SIMPLE RHEOLOGICAL METHOD FOR THE INVITRO ASSESSMENT OF MUCIN-POLYMER BIOADHESIVE BOND STRENGTH
A SIMPLE RHEOLOGICAL METHOD FOR THE INVITRO ASSESSMENT OF MUCIN-POLYMER BIOADHESIVE BOND STRENGTH
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DOI：
10.1023/a:1015812615635
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发表时间：
1990-05-01
影响因子：
3.7
通讯作者：
GALLO, JM
中科院分区：
文献类型：
--
作者：
HASSAN, EE;GALLO, JM
采用简单的粘度法定量测定黏液-聚合物生物粘合剂的结合强度。在选择中性、阴离子和阳离子聚合物（0.1-2.5%,w/v）不存在（. etam）或存在（. etam）的情况下，用Brookfield粘度计测量15% （w/v）猪胃粘蛋白分散体在0.1 N HCl （pH 1）或0.1 N醋酸盐缓冲液（pH 5.5）中的粘度。生物黏附的黏度组分（.eta.b）由方程。eta计算。T =。M +。P +。B，其中。p为用奥斯特瓦尔德粘度计测得的相应纯聚合物溶液的粘度。生物粘附力(F)由公式F = .eta.b.sigma计算。，其中。sigma。为剪切速率/秒。聚电解质（如聚丙烯酸、阳离子明胶和壳聚糖）的b′s和F′s在醋酸缓冲液中始终高于在盐酸中。该技术的有效性以及离子电荷、聚合物构象和剪切速率对。eta的影响。讨论了b和F，并将该方法与其他评价生物胶粘剂材料的方法进行了比较。
A simple viscometric method was used to quantify mucin-polymer bioadhesive bond strength. Viscosities of 15% (w/v) porcine gastric mucin dispersions in 0.1 N HCl (pH 1) or 0.1 N acetate buffer (pH 5.5) were measured with a Brookfield viscometer in the absence (.eta.m) or presence (.eta.t) of selected neutral, anionic, and cationic polymers (0.1-2.5%, w/v). Viscosity components of bioadhesion (.eta.b) were calculated from the equation, .eta.t = .eta.m + .eta.p + .eta.b, where .eta.p is the viscosity of corresponding pure polymer solution as measured by an Ostwald viscometer. The forces of bioadhesion (F) were calculated from the equation, F = .eta.b.sigma., where .sigma. is the rate of shear/sec. .eta.b''s and F''s for polyelectrolytes, e.g., polyacrylic acid, cationic gelatin, and chitosan were always higher in acetate buffer than in HCl. Validity of the technique and the effect of ionic charge, polymer conformation, and rate of shear on .eta.b and F are discussed, as is a comparison of this method to other methods for evaluating bioadhesive materials.