MCDONOUGH FELINE SARCOMA-VIRUS - CHARACTERIZATION OF THE MOLECULARLY CLONED PROVIRUS AND ITS FELINE ONCOGENE (V-FMS)
MCDONOUGH FELINE SARCOMA-VIRUS - CHARACTERIZATION OF THE MOLECULARLY CLONED PROVIRUS AND ITS FELINE ONCOGENE (V-FMS)
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DOI：
10.1128/jvi.41.2.489-500.1982
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发表时间：
1982-01-01
影响因子：
5.4
通讯作者：
SHERR, CJ
中科院分区：
文献类型：
--
作者：
DONNER, L;FEDELE, LA;SHERR, CJ
通过分子克隆、转化原病毒DNA的分析，推导了猫肉瘤病毒麦克多诺株（SM-FeSV）的遗传结构。SM-FeSV原病毒全长8.2千碱基对（Kbp），比其他猫肉瘤病毒长，含有一个转化基因（v-fms），两侧的序列来源于猫白血病病毒。基因相对于病毒RNA的顺序为5“-gag-fms-env-3”，其中代表了整个猫白血病病毒env基因和几乎完整的gag序列。用克隆的SM-FeSV前病毒DNA转染[小鼠]NIH/3T3细胞，诱导表达SM-FeSV基因产物的形态学转化细胞灶，并含有可拯救的肉瘤病毒基因组。经病毒感染或转染克隆的原病毒DNA后转化的细胞表达SM-FeSV株特征的多蛋白（P170gag-fms）。在代谢标记的转化细胞的免疫沉淀中也发现了两种蛋白水解裂解产物（P120fms和pp55gag）。在SM-FeSV转化体中检测到的另一种多肽水平相对较低，其大小和抗原性与猫白血病病毒的包膜前体（gPr85env）没有区别。v-fms基因的复杂性(3.1 .+-。0.3 kbp)是。比Snyder-Theilen和Gardner-Arnstein FeSV的病毒致癌基因序列（v-fes）高2倍。通过异双工、限制性内切酶和核酸杂交分析，v-fms和v-fes序列没有同源性。放射性标记的DNA片段代表了与正常猫细胞DNA的不同EcoRI和HindIII片段杂交的2个病毒癌基因的部分。与v-fms相关的细胞序列（称为c-fms）比c-fms复杂得多，在猫DNA中以节段分布在100 - 40 kbp之间。分子克隆的Snyder-Theilen、Gardner-Arnstein和SM-FeSV原病毒的比较结构研究表明，猫白血病病毒基因组中来自pol-env连接的区域在每个FeSV毒株中都与v-onc序列相邻，并且可能提供了与细胞基因重组的首选序列。
The genetic structure of the McDonough strain of feline sarcoma virus (SM-FeSV) was deduced by analysis of molecularly cloned, transforming proviral DNA. The 8.2-kilobase pair (Kbp) SM-FeSV provirus is longer than those of other feline sarcoma viruses and contains a transforming gene (v-fms) flanked by sequences derived from feline leukemia virus. The order of genes with respect to viral RNA is 5''-gag-fms-env-3'', in which the entire feline leukemia virus env gene and an almost complete gag sequence are represented. Transfection of [mouse] NIH/3T3 cells with cloned SM-FeSV proviral DNA induced foci of morphologically transformed cells which expressed SM-FeSV gene products and contained rescuable sarcoma viral genomes. Cells transformed by viral infection or after transfection with cloned proviral DNA expressed the polyprotein (P170gag-fms) characteristic of the SM-FeSV strain. Two proteolytic cleavage products (P120fms and pp55gag) were also found in immunoprecipitates from metabolically labeled, transformed cells. An additional polypeptide, detected at comparatively low levels in SM-FeSV transformants, was indistinguishable in size and antigenicity from the envelope precursor (gPr85env) of feline leukemia virus. The complexity of the v-fms gene (3.1 .+-. 0.3 kbp) is .apprx. 2-fold greater than the viral oncogene sequences (v-fes) of Snyder-Theilen and Gardner-Arnstein FeSV. By heteroduplex, restriction enzyme and nucleic acid hybridization analyses, v-fms and v-fes sequences showed no detectable homology to one another. Radiolabeled DNA fragments representing portions of the 2 viral oncogenes hybridized to different EcoRI and HindIII fragments of normal cat cellular DNA. Cellular sequences related to v-fms (designated c-fms) were much more complex than c-fes and were distributed segmentally over > 40 kbp in cat DNA. Comparative structural studies of the molecularly cloned proviruses of Snyder-Theilen, Gardner-Arnstein and SM-FeSV showed that a region of the feline leukemia virus genome derived from the pol-env junction is represented adjacent to v-onc sequences in each FeSV strain and may have provided sequences preferred for recombination with cellular genes.