MiR-135b promotes proliferation and invasion of osteosarcoma cells via targeting FOXO1
MiR-135b promotes proliferation and invasion of osteosarcoma cells via targeting FOXO1
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DOI：
10.1007/s11010-014-2281-2
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发表时间：
2015-02-01
影响因子：
4.3
通讯作者：
Guo, Weichun
中科院分区：
文献类型：
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作者：
Pei, Hong;Jin, Zhiliang;Guo, Weichun
最近的数据有力地表明了miRNAs在各种癌症相关过程中的重要作用。骨肉瘤(Osteosaroma，OS)是最常见的原发骨癌，因其快速增殖和转移而导致死亡。在这里，我们证明了与非癌骨组织相比，miR-135B在OS标本中的表达经常上调，与潜在的靶-FOXO1表达模式呈负相关。生物信息学分析结合实验证实FOXO1是OS中miR-135B的直接靶点。在功能上，miR-135B抑制剂显著抑制OS细胞的增殖和侵袭。强制表达FOXO1则表现出相反的作用，下调FOXO1可阻断miR-135B抑制剂的作用。综上所述，我们的数据提供了令人信服的证据，表明miR-135B在OS中作为癌基因-miRNA促进OS细胞的增殖和侵袭，其致癌作用主要通过靶向FOXO1介导。
Recent data strongly suggest the important role of miRNAs in various cancer-related processes. Osteosarcoma (OS) is the most common primary cancer of the bone and usually leads to deaths due to its rapid proliferation and metastasis. Here, we demonstrated that compared with noncancerous bone tissues, miR-135b expression is frequently upregulated in OS specimens, inversely correlated with potential target-FOXO1 expression pattern. Bioinformatics analysis combined with experimental confirmation revealed FOXO1 is a direct target of miR-135b in OS. Functionally, miR-135b inhibitor significantly inhibited OS cells proliferation and invasion. Forced expression of FOXO1 showed the opposite effect, and FOXO1 knockdown abolished the effect of miR-135b inhibitor. Taken together, our data provide compelling evidence that miR-135b functions as an onco-miRNA in OS to promote OS cells proliferation and invasion, and its oncogenic effects are mediated chiefly through targeting FOXO1.