MAP1B phosphorylation is differentially regulated by Cdk5/p35, Cdk5/p25, and JNK
MAP1B phosphorylation is differentially regulated by Cdk5/p35, Cdk5/p25, and JNK
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DOI：
10.1016/j.bbrc.2005.03.132
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发表时间：
2005-05-27
影响因子：
3.1
通讯作者：
Hoshino, M
中科院分区：
文献类型：
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作者：
Kawauchi, T;Chihama, K;Hoshino, M
模式I磷酸化的MAPK B在发育和致病的大脑中被观察到。虽然Cdk 5被认为在发育的大脑皮层中磷酸化MAPK B，但是我们表明Cdk 5抑制剂在原代和切片培养中不抑制模式I磷酸化的MAP 1 B，而JNK抑制剂则抑制。巧合的是，当Cdk 5与p35共转染时，在COS 7细胞中没有观察到磷酸化的MAP 1B的增加，但这确实发生在致病性脑中特异性产生的p25中。我们的原代培养研究表明，Cdk 5参与调节微管动力学，而不影响MAP 1B磷酸化状态。微管动力学调节在神经元迁移中的重要性也通过Urea电穿孔实验得到证实。这些发现表明，在发育中的大脑皮层中，JNK而不是Cdk 5/p35促进了MAP 1B的模式I磷酸化，而在致病性大脑中，则由Cdk 5/p25促进。导致了各种生物事件。(c)2005年爱思唯尔公司All rights reserved.
Mode I phosphorylated MAPI B is observed in developing and pathogenic brains, Although Cdk5 has been believed to phosphorylate MAPI B in the developing cerebral cortex, we show that it Cdk5 inhibitor does not suppress mode I phosphorylation of MAP1B in primary and slice cultures, while a JNK inhibitor does. Coincidently, in increase in phosphorylated MAP1B was not observed in COS7 cells when Cdk5 was cotransfected with p35, but this did occur with p25 which is specifically produced in pathogenic brains. Our primary culture studies showed an involvement of Cdk5 in regulating microtubule dynamics Without affecting MAP1B phosphorylation status. The importance of regulating microtubule dynamics in neoronal migration was also demonstrated by in Utero electroporation experiments, These findings Suggest that mode I phosphorylation of MAP1B is facilitated by JNK but not Cdk5/p35 in the developing cerebral cortex and by Cdk5/p25 in pathogenic brains. contributing to various biological events. (c) 2005 Elsevier Inc. All rights reserved.