Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.)
Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.)
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DOI：
10.1007/s001220050596
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发表时间：
1997-09-01
影响因子：
5.4
通讯作者：
McCouch, SR
中科院分区：
文献类型：
--
作者：
Chen, X;Temnykh, S;McCouch, SR
将94个新开发的微卫星标记整合到4个水稻群体的RFLP框架图中，包括两个双单倍体，一个重组自交系和一个种间回交群体。这些简单重复序列（SSR）主要是聚（GA）基序，因为它们在水稻中的丰度。它们是从先前描述的剪切文库和新构建的酶消化文库中分离的。从两个文库中分离的克隆观察到聚（GA）道的平均长度的差异。对于从Tsp-509消化的文库分离的克隆，GA基序的长度平均为21个重复单位，而对于从剪切的文库分离的克隆，基序的长度平均为17个单位。没有证据表明着丝粒或端粒附近的微卫星标记聚类。94个新开发的标记以及27个先前报道的微卫星的定位提供了12条染色体的全基因组覆盖，平均距离为每16-20 cM 1个SSLP（简单序列重复多态性）。
Ninety-four newly developed microsatellite markers were integrated into existing RFLP framework maps of four rice populations, including two doubled haploid, a recombinant inbred, and an interspecific backcross population. These simple sequence repeats (SSR) were predominantly poly(GA) motifs, targetted because of their abundance in rice. They were isolated from a previously described sheared library and a newly constructed enzyme-digested library. Differences in the average length of poly(GA) tracts were observed for clones isolated from the two libraries. The length of GA motifs averaged 21 repeat units for clones isolated from the Tsp-509-digested library, while motifs averaged 17 units for clones from the sheared library. There was no evidence of clustering of microsatellite markers near centromeres or telomeres. Mapping of the 94 newly developed markers as well as of 27 previously reported microsatellites provided genome-wide coverage of the 12 chromosomes, with an average distance of 1 SSLP (simple sequence repeat polymorphism) per 16-20 cM.