An early decrease in phosphatidylinositol turnover occurs on induction of Friend cell differentiation and precedes the decrease in c-myc expression.
An early decrease in phosphatidylinositol turnover occurs on induction of Friend cell differentiation and precedes the decrease in c-myc expression.
复制标题
磷脂酰肌醇周转的早期减少发生在 Friend 细胞分化诱导时，并且先于 c-myc 表达的减少。
DOI：
10.1016/0092-8674(85)90037-6
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发表时间：
1985
期刊：
影响因子：
64.5
通讯作者：
Macara,IG
中科院分区：
文献类型：
--
作者：
Faletto,DL;Arrow,AS;Macara,IG
磷脂酰肌醇的代谢最近被认为与增殖和转化的调节有关。目前已经用Friend红白血病细胞研究了它在分化中的作用，二甲基亚砜和某些其他药物可以诱导红系细胞沿红系途径分化。我们发现，在Friend细胞诱导分化的2小时内，磷脂酰肌醇代谢物三磷酸肌醇和乳酸甘油的水平显著下降。这些减少降低了c-myc原癌基因及其蛋白产物的表达。佛波醇11-肉豆蔻酸酯，13-乙酸酯可以模拟二酰甘油，在不逆转磷脂酰肌醇代谢物水平下降的情况下阻止分化。两个人工合成的二酰甘油，La-1-油酰基-2-乙酰-SN-3-甘油和SN-1，2-二辛酰甘油也阻碍了分化和承诺。二酰甘油对激酶C活性的调节可能在控制c-myc表达和Friend细胞分化中起关键作用。
Phosphatidylinositol turnover has recently been implicated in the regulation of proliferation and transformation. Its role in differentiation has now been investigated using Friend erythroleukemia cells, which can be induced to differentiate along the erythroid pathway by dimethylsulfoxide and certain other agents. We have found that levels of the phosphatidylinositol metabolites inositol-trisphosphate and dlacylglycerol significantly decrease within 2 hr of Induction of Friend cell differentiation. These decreases pmcede decreased expression of the c-myc proto-oncogene and its protein product. Phorbol ll-myristate, 13-acetate, which can mimic diacylglycerol, blocked differentiation without reversing the decrease in phosphatidylinositol metabolite levels. Two synthetic diacylglycerols, La-1-oleoyl-2-acetoyl-sn-3-glycerol and sn-1, 2-dioctanoylglycerol, also blocked differentiation and commitment. Diacylglycerol regulation of kinase C activity may play a key role in control of c-myc expression and Friend cell differentiation.