Concurrent measurement of microbiome and allergens in the air of bedrooms of allergy disease patients in the Chicago area
Concurrent measurement of microbiome and allergens in the air of bedrooms of allergy disease patients in the Chicago area
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DOI：
10.1186/s40168-019-0695-5
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发表时间：
2019-06-03
期刊：
影响因子：
15.5
通讯作者：
Hampton-Marcell, Jarrad T.
中科院分区：
文献类型：
--
作者：
Richardson, Miles;Gottel, Neil;Hampton-Marcell, Jarrad T.
室内空气中的颗粒物和生物成分对人体健康有很大的影响，特别是免疫呼吸系统疾病，如哮喘。为了更好地探索过敏原，微生物群落和室内生活环境之间的关系，我们在芝加哥地区的65个家庭的卧室进行了采样，使用23患者友好的Inspirtec电动空气采样设备，收集空气中的颗粒，用于表征过敏原和微生物DNA。采样装置捕获了足够的微生物材料，以使研究中的每份样本能够生成16 S rRNA扩增子测序数据。HEPA过滤器的存在和空气采样装置放置的高度对微生物群落分布都没有任何影响。超过四分之三的样本中存在31个OTU的核心微生物群，占每个卧室中相对序列计数的45%左右。最丰富的单一生物是葡萄球菌，其他核心类群都是人类和户外相关的。细菌α多样性在窗户开着的卧室、附近有开花植物的卧室和有狗的房间里显著增加。卟啉单胞菌属、莫拉氏菌属、萨特雷氏菌属和梭菌属以及奈瑟氏菌科沿着在有狗的家庭中显著富集;有趣的是，猫对微生物多样性或相对丰度没有显着影响。虽然狗过敏原负荷与细菌α多样性显着相关，但与过敏原负荷显着相关的分类群并不完全与家中养狗的那些丰富的分类群重叠。链格孢菌变应原负荷与细菌α多样性呈正相关，而曲霉变应原负荷呈负相关。链格孢变应原负荷也与打开的窗户显著相关。微生物群落在农村、郊区和城市家庭之间存在显著差异，并且物理上彼此靠近的房屋保持了更相似的微生物群。我们已经证明，可以从同一样本中确定过敏原负荷与空气中微生物群之间的显著关联，并且这些关联与家庭和社区的特征有关。
The particulate and biological components of indoor air have a substantial impact on human health, especially immune respiratory conditions such as asthma. To better explore the relationship between allergens, the microbial community, and the indoor living environment, we sampled the bedrooms of 65 homes in the Chicago area using 23the patient-friendly Inspirotec electrokinetic air sampling device, which collects airborne particles for characterization of both allergens and microbial DNA. The sampling device captured sufficient microbial material to enable 16S rRNA amplicon sequencing data to be generated for every sample in the study. Neither the presence of HEPA filters nor the height at which the air sampling device was placed had any influence on the microbial community profile. A core microbiota of 31 OTUs was present in more than three quarters of the samples, comprising around 45% of the relative sequence counts in each bedroom. The most abundant single organisms were Staphylococcus, with other core taxa both human and outdoor-associated. Bacterial alpha diversity was significantly increased in bedrooms that reported having open windows, those with flowering plants in the vicinity, and those in homes occupied by dogs. Porphyromonas, Moraxella, Sutterella, and Clostridium, along with family Neisseraceae, were significantly enriched in homes with dogs; interestingly, cats did not show a significant impact on microbial diversity or relative abundance. While dog allergen load was significantly correlated with bacterial alpha diversity, the taxa that significantly correlated with allergen burden did not exclusively overlap with those enriched in homes with dogs. Alternaria allergen load was positively correlated with bacterial alpha diversity, while Aspergillus allergen load was negatively correlated. The Alternaria allergen load was also significantly correlated with open windows. Microbial communities were significantly differentiated between rural, suburban, and urban homes and houses that were physically closer to each other maintained significantly more similar microbiota. We have demonstrated that it is possible to determine significant associations between allergen burden and the microbiota in air from the same sample and that these associations relate to the characteristics of the home and neighborhoods.