Ethylene signaling increases reactive oxygen species accumulation to drive root hair initiation in Arabidopsis
Ethylene signaling increases reactive oxygen species accumulation to drive root hair initiation in Arabidopsis
复制标题
乙烯信号增加活性氧积累，驱动拟南芥根毛形成
DOI：
10.1242/dev.200487
复制
发表时间：
2022-07-01
期刊：
影响因子：
4.6
通讯作者：
Muday, Gloria K.
中科院分区：
文献类型：
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作者：
Martin, R. Emily;Marzol, Eliana;Muday, Gloria K.
根毛发生是根发育的一个高度调节的方面。植物激素乙烯及其前体1-氨基-环丙烷-1-羧酸诱导根毛的形成和伸长。使用共聚焦显微镜与氧化还原生物传感器和染料配对，我们证明了提高乙烯水平的治疗导致过氧化氢积累在毛细胞之前，根毛形成。在乙烯不敏感受体突变体etr 1 - 3和信号双突变体ein 3eil 1中，ACC和乙烯处理后根毛数量或活性氧（ROS）积累的增加消失。相反，etr 1 - 7，组成型乙烯信号受体突变体，增加了根毛的形成和活性氧积累，类似于乙烯处理的Col-0幼苗。随想转录因子突变体和狼人转录因子突变体分别降低和升高了活性氧水平，这与根毛起始水平相关。rhd 2 6突变体，与缺陷的基因编码的ROS-合成的爆发氧化酶同源异型C（RBOHC），和prx 44 - 2突变体，这是缺陷的III类过氧化物酶，表现出受损的乙烯依赖性的ROS合成和根毛形成通过EIN 3EIL 1依赖性的转录调控。总之，这些结果表明乙烯通过RBOHC和PRX44增加ROS积累以驱动根毛形成。
Root hair initiation is a highly regulated aspect of root development. The plant hormone ethylene and its precursor, 1-amino-cyclopropane-1-carboxylic acid, induce formation and elongation of root hairs. Using confocal microscopy paired with redox biosensors and dyes, we demonstrated that treatments that elevate ethylene levels lead to increased hydrogen peroxide accumulation in hair cells prior to root hair formation. In the ethylene-insensitive receptor mutant, etr1-3, and the signaling double mutant, ein3eil1, the increase in root hair number or reactive oxygen species (ROS) accumulation after ACC and ethylene treatment was lost. Conversely, etr1-7, a constitutive ethylene signaling receptor mutant, has increased root hair formation and ROS accumulation, similar to ethylene-treated Col-0 seedlings. The caprice and werewolf transcription factor mutants have decreased and elevated ROS levels, respectively, which are correlated with levels of root hair initiation. The rhd2 6 mutant, with a defect in the gene encoding the ROS-synthesizing RESPIRATORY BURST OXIDASE HOMOLOG C (RBOHC), and the prx44-2 mutant, which is defective in a class III peroxidase, showed impaired ethylene-dependent ROS synthesis and root hair formation via EIN3EIL1 -dependent transcriptional regulation. Together, these results indicate that ethylene increases ROS accumulation through RBOHC and PRX44 to drive root hair formation.