Evaluation and identification of potential organic nematicidal volatiles from soil bacteria
Evaluation and identification of potential organic nematicidal volatiles from soil bacteria
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DOI：
10.1016/j.soilbio.2007.05.011
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发表时间：
2007-10-01
影响因子：
9.7
通讯作者：
Zhang, Ke-Qin
中科院分区：
文献类型：
--
作者：
Gu, Ying-Qi;Mo, Ming-He;Zhang, Ke-Qin
天然存在的具有杀线虫活性的挥发性化合物（NAs）在农业和林业中具有重要的经济意义。本研究对200株土壤细菌产生的挥发性有机物进行了体外实验研究。我们的研究结果表明，在200个细菌分离物，149（74.5%）和165（82.5%）表现出大于20%的NA对自由生活的线虫Panagrellus redivus和松材线虫，分别。其中22个菌株对P. redivus的NA为100%，7个菌株对B的NA为100%。xYlophilus。当接触杀线虫挥发物时，线虫在处理后1-12小时内逐渐减少运动，大多数在24小时后完全停止运动。我们的分析表明，不仅在细菌物种之间，而且在同一物种的分离株之间的挥发性有机化合物的杀线虫能力的显着变化。采用固相微萃取（SPME）技术提取代表性分离物的挥发性成分，气相色谱-质谱（GC-MS）联用技术鉴定其结构。检出的挥发性化合物包括醇类、醛类、酮类、烯烃类和醚类。在具有强NA（>~ 80%）的20种VOC中，9种（苯酚、2-辛醇、苯甲醛、苯乙醛、癸醛、2-壬酮、2-十一烷酮、环己烯和二甲基二硫醚）对两种模型线虫显示100% NA。此外，松油醇、苯乙醇、丙酮、苯乙酮和壬烷等5种化合物的NA与B不同。松材线虫（75-100%）和红腐霉（17.02-100%）。((c)2007爱思唯尔有限公司保留所有权利。
Naturally occurring volatile compounds with nematicidal activities (NAs) are of significant economical importance in agriculture and forestry. In this study, volatile organic compounds (VOCs) produced by 200 isolates of soil bacterial were evaluated in in vitro experiments. Our results identified that among the 200 bacterial isolates, 149 (74.5%) and 165 (82.5%) exhibited a greater than 20% NA against the free-living nematode Panagrellus redivivus and the pinewood nematode Bursaphelenchus xylophilus, respectively. Among them, 22 isolates showed 100% NA against P. redivivus and seven isolates showed 100% NA against B. xYlophilus. When exposed to nernaticidal volatiles, nematodes gradually reduced their movements within 1-12 h after treatment, and most stopped moving completely after 24 h. Our analysis indicated significant variation in nernaticidal capability of the VOCs not only among bacterial species but also among isolates of the same species. Volatiles of representative isolates were extracted using solid-phase micro-extraction (SPME) and their structures identified using gas chromatography-mass spectrometry (GC-MS). The detected so volatile compounds included alcohols, aldehydes, ketones, alkenes and ethers. Of the 20 VOCs with strong NA (>-80%), nine (phenol, 2-octanol, benzaldehyde, benzeneacetaidehyde, decanal, 2-nonanone, 2-undecanone, cyclohexene and dimethyl disulfide) displayed 100% NA to both model nematodes. Furthermore, five compounds (terpineol, benzeneethanol, propanone, phenyl ethanone and nonane) showed different NA to B. xylophilus (75-100%) and P. redivivus (17.02-100%). ((c) 2007 Elsevier Ltd. All rights reserved.