Untargeted LC–MS metabolomics approach reveals metabolic changes in genetically improved farmed tilapia (Oreochromis niloticus) with fatty liver induced by a high‐fat diet
Untargeted LC–MS metabolomics approach reveals metabolic changes in genetically improved farmed tilapia (Oreochromis niloticus) with fatty liver induced by a high‐fat diet
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DOI：
10.1111/are.14929
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发表时间：
2020-10
影响因子：
2
通讯作者：
Yifan Tao;J. Qiang;Jie He;Hao-Jun Zhu;Jingwen Bao;P. Xu
中科院分区：
文献类型：
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作者：
Yifan Tao;J. Qiang;Jie He;Hao-Jun Zhu;Jingwen Bao;P. Xu
肝脏脂肪变性通常发生在集约化养殖的罗非鱼中。这种疾病对鱼类的生长和健康是有害的，但对脂肪肝罗非鱼代谢变化的了解有限。在本研究中，我们使用基于LC-MS的肝脏代谢组学分析和传统营养评估，比较了饲喂高脂饲料(HFD)和普通脂肪饲料(NFD)8周的雄性遗传改良养殖罗非鱼(GIFT，Oreochromis niloticus)。喂食高脂饮食的青少年比喂食NFD的婴儿在肝脏中积累的脂肪更多。代谢组学分析显示，两组之间存在36种不同积累的代谢物，这些代谢物参与了25条信号通路。我们的结果表明，脂肪肝的发展严重损害了肝脏的脂质代谢，导致TCA循环受阻，进一步扰乱了GIFT中的蛋白质和碳水化合物代谢。这些生理变化可能与饲喂高脂饲料的小鼠生长速度较慢有关。总体而言，我们的结果揭示了高脂饲料喂养的GIFT中代谢紊乱的细节，并增强了我们对GIFT中脂肪肝形成机制的了解。
Hepatic steatosis commonly occurs in intensively farmed tilapia. This disease is harmful to fish growth and health, but knowledge of the metabolic changes in tilapia with fatty liver is limited. In the present study, we compared male genetically improved farmed tilapia (GIFT,Oreochromis niloticus) fed a high‐fat diet (HFD) with those fed a normal‐fat diet (NFD) for 8 weeks using LC–MS‐based hepatic metabolomic assays and traditional nutritional assessments. Juvenile GIFT fed a HFD accumulated more fat in the liver than did those fed a NFD. The metabolomic analyses revealed 36 differentially accumulated metabolites between the two groups, and these metabolites were involved in 25 signalling pathways. Our results indicate that development of fatty liver severely impairs hepatic lipid metabolism, leading to blockage of the TCA cycle and further disruptions to protein and carbohydrate metabolism in GIFT. These physiological changes may be related to the lower growth rate of GIFT fed a HFD. Overall, our results reveal the details of metabolic disorders in GIFT fed a HFD and enhance our knowledge of the mechanism of fatty liver formation in GIFT.