An ultrasensitive and ultraselective chemiluminescence aptasensor for dopamine detection based on aptamers modified magnetic mesoporous silica @ graphite oxide polymers
An ultrasensitive and ultraselective chemiluminescence aptasensor for dopamine detection based on aptamers modified magnetic mesoporous silica @ graphite oxide polymers
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DOI：
10.1016/j.snb.2017.10.171
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发表时间：
2018-03
影响因子：
8.4
通讯作者：
Yuanling Sun;Yanna Lin;Chaofan Ding;Weiyan Sun;Yuxue Dai;Xiaodong Zhu;Hao Liu;Chuannan Luo
中科院分区：
文献类型：
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作者：
Yuanling Sun;Yanna Lin;Chaofan Ding;Weiyan Sun;Yuxue Dai;Xiaodong Zhu;Hao Liu;Chuannan Luo
本文以磁性介孔氧化硅@氧化石墨聚合物（Fe 3 O 4·mSiO 2·nSiO2@GO）为载体，制备了一种用于多巴胺（DA）检测的超灵敏、超选择性化学发光（CL）适体。首先，制备了Fe 3 O 4·mSiO 2·nSiO2@GO和Au纳米粒子（AuNPs），并通过透射电子显微镜（TEM）、扫描电子显微镜（SEM）、傅立叶变换红外光谱（FTIR）、X射线粉末衍射（XRD）等手段对其进行了表征。然后，将多巴胺适体（D-Apt）固定在Fe 3 O 4·mSiO 2·nSiO2@GO表面，并用ssDNA（与D-Apt部分互补的单链DNA）修饰金纳米粒子。研究了Fe 3 O 4·mSiO 2·nSiO2@GO对D-Apt的固定化性能和Fe 3 O 4·mSiO 2·nSiO 2 @GO@D-Apt对AuNPs@ssDNA的吸附性能。当DA存在时，AuNPs@ssDNA从Fe_3O_4·mSiO_2·nSiO_2 @GO@D-Atp表面脱附并催化发光。在优化的化学发光条件下，DA的线性范围为2.5 × 10− 13 ~ 2.0 × 10− 9 mol/L。方法的检出限为3.9 × 10 - 14 mol/L（3δ），相对标准偏差（RSD）为2.6%。最后，将Fe 3 O 4·mSiO 2·nSiO 2 @GO@D-Apt/AuNPs@ssDNA − CL适体传感器用于实际样品中DA的测定，回收率为98.0%~ 102.0%。这些令人满意的结果表明，所提出的CL适体传感器实现了超选择性，超灵敏度和可靠的检测DA，并揭示了潜在的应用于监测和诊断人类神经系统疾病。
In this work, an ultrasensitive and ultraselective chemiluminescence (CL) aptasensor was prepared for dopamine (DA) detection based on aptamers modified magnetic mesoporous silica @ graphite oxide polymers (Fe3O4·mSiO2·nSiO2@GO). Firstly, Fe3O4·mSiO2·nSiO2@GO and Au nanoparticles (AuNPs) were prepared and characterizated by transmission electron microscopy (TEM), scanning electron microscope (SEM), fourier transform infrared (FTIR), X-ray powder diffraction (XRD) and others. Then, dopamine aptamer (D-Apt) was immobilized on the surface of Fe3O4·mSiO2·nSiO2@GO while AuNPs was modified by ssDNA (a single stranded DNA partially complementary to D-Apt). The immobilization properties of Fe3O4·mSiO2·nSiO2@GO to D-Apt and the adsorption properties of Fe3O4·mSiO2·nSiO2@GO@D-Apt to AuNPs@ssDNA were researched sequentially. When DA existed, AuNPs@ssDNA was desorbed from the surface of Fe3O4·mSiO2·nSiO2@GO@D-Atp and catalyzed luminescence. After that, under optimized CL conditions, DA could be measured with the linear concentration range of 2.5 × 10−13to 2.0 × 10−9mol/L. The detection limit was 3.9 × 10−14mol/L (3δ) while the relative standard deviation (RSD) was 2.6%. Finally, the Fe3O4·mSiO2·nSiO2@GO@D-Apt/AuNPs@ssDNA − CL aptasensor was used for the determination of DA in practical samples and recoveries ranged from 98.0% to 102.0%. Those satisfactory results clarified the proposed CL aptasensor achieved ultraselective, ultrasensitive and reliable detection of DA and revealed potential application in monitoring and diagnosis of human neurological diseases.