Characterization of lactobacilli towards their use as probiotic adjuncts in poultry
Characterization of lactobacilli towards their use as probiotic adjuncts in poultry
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DOI：
10.1046/j.1365-2672.2002.01608.x
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发表时间：
2002-01-01
影响因子：
4
通讯作者：
Vogel, RF
中科院分区：
文献类型：
--
作者：
Ehrmann, MA;Kurzak, P;Vogel, RF
目的：共112株乳酸菌的鸭源进行了研究，他们作为一个益生菌饲料supply.Methods和结果：在体外研究包括聚集，共聚集，细胞表面疏水性和家禽作物细胞和人类Hep2细胞上的粘附活性。此外，还测试了胆汁酸（鸡胆汁、牛胆和牛磺胆酸）的生长和对酸性pH的耐受性。在所有分离物中，选择两个菌株（动物乳杆菌TMW 1.972和唾液乳杆菌TMW 1.992）用于家禽存活试验。通过选择性检测利福平和红霉素双耐药突变体，实现了对这些菌株的监测和区分。单次投喂后，这两种微生物分别在鸭的作物和盲肠中持续存在18天和22天。为了确认Lact的身份。animalis和Lact.结论：在鸭源性微生物群中，两株乳酸杆菌具有较强的益生菌潜力。结果表明，家禽的天然肠道微生物区系是最佳菌株的良好来源。研究的意义和影响：提出了选择益生菌菌株的一般策略。所建议的测试顺序允许在复杂的生态系统或大型菌株集合中以最小的支出确定最有希望的候选物。
Aims: A total of 112 strains of lactic acid bacteria of duck origin were studied for their use as a probiotic feed supplement.Methods and Results: In vitro studies included aggregation, co-aggregation, cell surface hydrophobicity and adhesion activities on poultry crop cells and human Hep2-cells. Additionally, growth with bile acids (chicken bile, ox gall and taurocholic acid) and tolerance to acidic pH were tested. Among all the isolates, two strains (Lactobacillus animalis TMW 1.972 and Lactobacillus salivarius TMW 1.992) were selected for a survival test in poultry. Monitoring and differentiation of these strains was achieved by selective detection as rifampicin and erythromycin double-resistant mutants. After a single feed administration, both micro-organisms were shown to persist in the crop and caecum of ducks for a period of 18 and 22 days, respectively. For identification of Lact. animalis and Lact. salivarius , two specific PCRs targeted against 16S rDNA were developed.Conclusions: Within the autochtoneous microflora of ducks, two strains of lactobacilli exhibited strong potential as probiotic adjuncts. The results indicate that the natural gut microflora of poultry serves as an excellent source for optimal strains.Significance and Impact of the Study: A general strategy for the selection of probiotic strains is presented. The suggested sequence of tests allows identification of the most promising candidates within complex ecosystems or large strain collections with minimal expenditure.