Social defeat stress induces phosphorylation of extracellular signal-regulated kinase in the leptomeninges in mice
Social defeat stress induces phosphorylation of extracellular signal-regulated kinase in the leptomeninges in mice
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DOI：
10.1002/npr2.12051
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发表时间：
2019-06-01
影响因子：
2.5
通讯作者：
Furuyashiki, Tomoyuki
中科院分区：
文献类型：
--
作者：
Okamura, Satoshi;Nagai, Hirotaka;Furuyashiki, Tomoyuki
目的：使用各种压力模型的动物研究表明，过度的环境压力会诱发抑郁症？以及通过大脑和外周炎症反应产生的焦虑样行为。尽管软脑膜细胞具有与大脑炎症反应相关的多种功能，但环境压力是否影响软脑膜仍不清楚。在本研究中，我们旨在检测软脑膜中细胞外信号调节激酶（ERK）的磷酸化。方法：我们让 C57BL/6 雄性小鼠经历单次社交失败应激，并通过免疫组织化学分析软脑膜中磷酸化 ERK 的表达。结果：小鼠的社交失败应激诱导软脑膜中靠近血管内皮细胞和胶质细胞的 ERK 磷酸化。这种ERK磷酸化在应激后维持至少一小时。结论：这项研究首次显示了环境应激对软脑膜的影响，并为阐明其在应激引起的神经功能变化中的功能作用铺平了道路。
Aims: Animal studies using various stress models have shown that excessive environmental stress induces depression? and anxiety-like behaviors through inflammatory responses in the brain and periphery. Although the leptomeningeal cells have multiple functions related to inflammatory responses in the brain, whether environmental stress influences the leptomeninges remains unknown. In this study, we aimed to examine phosphorylation of the extracellular signal-regulated kinase (ERK) in the leptomeninges.Methods: We subjected C57BL/6 male mice to a single episode of social defeat stress and analyzed the expression of phosphorylated ERK in the leptomeninges by immunohistochemistry.Results: Social defeat stress in mice induced phosphorylation of ERK in the leptomeninges, adjacent to vascular endothelial cells and the glia limitans. This ERK phosphorylation was maintained for at least one hour after the stress.Conclusions: This study shows the effect of environmental stress on the leptomeninges for the first time and paves the way for elucidating its functional role in stress-induced changes in neural functions.