Mimicking peroxidase activity of Co2(OH)2CO3-CeO2 nanocomposite for smartphone based detection of tumor marker using paper-based microfluidic immunodevice
Mimicking peroxidase activity of Co2(OH)2CO3-CeO2 nanocomposite for smartphone based detection of tumor marker using paper-based microfluidic immunodevice
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DOI：
10.1016/j.talanta.2018.06.034
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发表时间：
2018-11-01
期刊：
影响因子：
6.1
通讯作者：
Hallaj, Rahman
中科院分区：
文献类型：
--
作者：
Alizadeh, Negar;Salimi, Abdollah;Hallaj, Rahman
本文报道了一种基于纸基微流控比色免疫传感器，该传感器采用具有特异性过氧化物酶活性的Co-2（OH）（2）CO 3-CeO 2纳米复合材料，用于检测癌胚抗原（CEA）。采用扫描电子显微镜（SEM）、透射电子显微镜（TEM）、X射线衍射（XRD）和X射线光电子能谱（XPS）对纳米复合材料的形貌和组成进行了表征。将离子液体和壳聚糖功能化的一抗（Ab（1））混合物负载于纸表面，可方便地制备免疫传感器。与传统的纸基免疫装置相比，当使用离子液体时，更有效地从纸表面去除非特异性结合蛋白。将二抗（Ab（2））堆叠在羧基化的Co-2（OH）（2）CO 3-CeO 2纳米复合材料的表面上。免疫传感器响应通过由Co-2（OH）（2）CO 3-CeO 2纳米复合物催化3，3 '，5，5'-四甲基联苯胺在H2 O2存在下的氧化而产生的颜色变化来获得。比色传感是在纸上完成的，使用智能手机拍照，然后用安装的应用程序分析颜色。本法测定CEA的线性范围为0.002 ~ 75.0 ng/mL，检出限为0.51 pg/mL。在本文中，我们开发了简单，具有成本效益和便携式设计的灵敏免疫测定和即时诊断癌症标志物。
We present a paper-based microfluidic colorimetric immunosensor for the detection of carcinoembryonic antigen (CEA), using Co-2(OH)(2)CO3-CeO2 nanocomposite with extraordinary intrinsic peroxidase like activity. The morphology and composition of the nanocomposite characterized with Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) techniques. The proposed immunosensor facilely fabricated by loading mixture of ionic liquid and chitosan functionalized primary antibodies (Ab(1)) on the surface of paper. Compared to traditional paper based immunodevice, when ionic liquid was used the nonspecific binding protein from the paper surface was more effectively removed. Secondary antibodies (Ab(2)) were stacked on the surface of the carboxylated Co-2(OH)(2)CO3-CeO2 nanocomposite. The immunosensor response was obtained by a color change resulting from Co-2(OH)(2)CO3-CeO2 nanocomposite catalyzing the oxidation of 3,3',5,5'-tetramethyl benzidine in the presence of H2O2. The colorimetric sensing was accomplished on the paper, using smartphone for taking a photo and then analyzing the colors with an installed application. Detection of CEA was performed by this method with a linear range from 0.002 to 75.0 ng mL(-1) and a detection limit of 0.51 pg mL(-1). In this paper we developed simple, cost-effective and portable design for sensitive immunoassay and point-of-care diagnostics of cancer marker.