Non-Covalently Associated Streptavidin Multi-Arm Nanohubs Exhibit Mechanical and Thermal Stability in Cross-Linked Protein-Network Materials.
Non-Covalently Associated Streptavidin Multi-Arm Nanohubs Exhibit Mechanical and Thermal Stability in Cross-Linked Protein-Network Materials.
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DOI：
10.1021/acs.biomac.2c00544
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发表时间：
2022-10-10
影响因子：
6.2
通讯作者：
Kim, Minkyu
中科院分区：
文献类型：
--
作者：
Knoff, David S.;Kim, Samuel;Cortes, Kareen A. Fajardo;Rivera, Jocelyne;Cathey, Marcus V. J.;Altamirano, Dallas;Camp, Christopher;Kim, Minkyu
构建具有单个蛋白质成分的物理化学和机械特性的蛋白质网络材料需要具有特异性和稳定性的分子交联剂。一个众所周知的例子涉及四臂聚乙二醇（tetra-PEG）与具有二级交联剂的所需蛋白质的特定化学融合。然而，有必要研究与蛋白质基因融合的四聚乙二醇类生物分子交联剂，通过消除额外的缀合和纯化步骤来简化合成。非共价、自缔合的链霉亲和素同四聚体是四聚乙二醇的可行的生物分子替代品。在这里，多臂链霉亲和素设计的特征是使用各种二级生物分子交联剂的蛋白质网络材料平台，例如高亲和力物理（即非共价）、瞬时物理、自发化学（即共价）或刺激诱导的化学交联剂。刺激诱导的化学交联剂与多臂链霉亲和素纳米中心融合，在引发永久共价键之前提供足够的扩散，从而能够正确表征链霉亲和素纳米中心。令人惊讶的是，非共价结合的链霉亲和素纳米中心表现出极高的稳定性，这转化为类似于化学键形成的水凝胶的材料特性，即使在高温下也是如此。因此，这项研究不仅证明了链霉亲和素纳米中心是一种理想的多臂生物聚合物前体，而且还为设计自组装纳米结构分子网络提供了宝贵的指导，该网络可以正确利用基于蛋白质的构建模块的非凡特性。
Constructing protein-network materials that exhibit physicochemical and mechanical properties of individual protein constituents requires molecular cross-linkers with specificity and stability. A well-known example involves specific chemical fusion of a four-arm polyethylene glycol (tetra-PEG) to desired proteins with secondary cross-linkers. However, it is necessary to investigate tetra-PEG like biomolecular cross-linkers that are genetically fused to the proteins, simplifying synthesis by removing additional conjugation and purification steps. Non-covalently, self-associating, streptavidin homotetramer is a viable, biomolecular alternative to tetra-PEG. Here, a multi-arm streptavidin design is characterized as a protein-network material platform using various secondary, biomolecular cross-linkers, such as high-affinity physical (i.e., non-covalent), transient physical, spontaneous chemical (i.e., covalent), or stimuli-induced chemical cross-linkers. Stimuli-induced, chemical cross-linkers fused to multi-arm streptavidin nanohubs provide sufficient diffusion prior to initiating permanent covalent bonds, allowing proper characterization of streptavidin nanohubs. Surprisingly, non-covalently associated streptavidin nanohubs exhibit extreme stability which translates into material properties that resemble hydrogels formed by chemical bonds even at high temperatures. Therefore, this study not only establishes that the streptavidin nanohub is an ideal multi-arm biopolymer precursor but also provides valuable guidance for designing self-assembling nanostructured molecular networks that can properly harness the extraordinary properties of protein-based building blocks.
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影响因子：
5.8
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Lust ST;Hoogland D;Norman MDA;Kerins C;Omar J;Jowett GM;Yu TTL;Yan Z;Xu JZ;Marciano D;da Silva RMP;Dreiss CA;Lamata P;Shipley RJ;Gentleman E
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