Reduction of the fertilizing capacity of sea urchin sperm by cannabinoids derived from marihuana. I. Inhibition of the acrosome reaction induced by egg jelly
Reduction of the fertilizing capacity of sea urchin sperm by cannabinoids derived from marihuana. I. Inhibition of the acrosome reaction induced by egg jelly
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大麻中提取的大麻素会降低海胆精子的受精能力。
DOI：
--
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发表时间：
1991
影响因子：
2.5
通讯作者：
S. Zimmerman
中科院分区：
文献类型：
--
作者：
H. Schuel;D. Berkery;R. Schuel;Michael C. Chang;A. Zimmerman;S. Zimmerman
Δ9-四氢大麻酚（THC）和来自大麻的其他两种主要大麻素-大麻二酚（CBD）和大麻酚（CBN）-通过降低精子的受精能力来抑制海胆中的受精（Schuel等人，1987年）。精子的生育能力取决于它们的活力和它们在遇到卵子的胶状物时进行顶体反应的能力。S.含有四氢大麻酚的purpuratus精子以剂量（0.1-100 μM）和时间（0-5 min）依赖性方式防止溶解的卵冻触发顶体反应。在用100 μM THC预处理5 min的精子中，88.9±2.3%的顶体反应诱导受到抑制，而与溶剂（溶剂）和海水处理的对照相比，THC处理的精子的活力没有降低。用6.6 μM THC预处理5 min和100 μM THC预处理20.8 sec后，顶体反应抑制50%。CBN和CBD在相当的浓度下以类似于THC的方式抑制卵冻的顶体反应。THC不抑制由离子霉素（促进Ca 2+内流）和尼日利亚菌素（促进K+外流）人工诱导的顶体反应。THC部分抑制（20-30%）由A23187诱导的顶体反应，其促进Ca 2+内流，和NH 4 OH，其提高精子的内部pH。添加莫能菌素，促进Na+流入卵冻或A23187，不克服THC抑制。THC对卵冻诱导的顶体反应的抑制会导致精子的受精能力相应降低。THC对顶体反应和精子生育力的不利影响是可逆的。这些发现表明，大麻素通过抑制卵冻诱导顶体反应来降低海胆精子的受精能力。THC可能影响离子通道开放之前的刺激-分泌偶联机制中的事件。
Δ9‐Tetrahydrocannabinol (THC) and two other major cannabinoids derived from marihuana–cannabidiol (CBD) and cannabinol (CBN)–inhibit fertilization in the sea urchin Strongylocentrotus purpuratus by reducing the fertilizing capacity of sperm (Schuel et al., 1987). Sperm fertility depends on their motility and on their ability to undergo the acrosome reaction upon encountering the egg's jelly coat. Pretreatment of S. purpuratus sperm with THC prevents triggering of the acrosome reaction by solubilized egg jelly in a dose (0.1–100 μM) and time (0–5 min)‐dependent manner. Induction of the acrosome reaction is inhibited in 88.9±2.3% of sperm pretreated with 100 μM THC for 5 min, while motility of THC‐treated sperm is not reduced compared to solvent (vehicle) and seawater‐treated controls. The acrosome reaction is inhibited 50% by pretreatment with 6.6 μM THC for 5 min and with 100 μM THC after 20.8 sec. CBN and CBD at comparable concentrations inhibit the acrosome reaction by egg jelly in a manner similar to THC. THC does not inhibit the acrosome reaction artificially induced by ionomycin, which promotes Ca2+ influx, and nigericin, which promotes K+ efflux. THC partially inhibits (20–30%) the acrosome reaction induced by A23187, which promotes Ca2+ influx, and NH4OH, which raises the internal pH of the sperm. Addition of monensin, which promotes Na+ influx to egg jelly or to A23187, does not overcome the THC inhibition. Inhibition of the egg jelly‐induced acrosome reaction by THC produces a corresponding reduction in the fertilizing capacity of the sperm. The adverse effects of THC on the acrosome reaction and sperm fertility are reversible. These findings show that cannabinoids reduce the fertilizing capacity of sea urchin sperm by inhibiting the induction of the acrosome reaction by egg jelly. THC may affect events in the stimulus–secretion coupling mechanism before the opening of ion channels.
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speract 和莫能菌素对海胆精子的钠依赖性激活。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hansbrough,JR;Garbers,DL
通讯作者：
Garbers,DL
DOI：
10.1016/s0021-9258(18)45597-4
发表时间：
1987-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
H. Farach;D. Mundy;W. Strittmatter;W. Lennarz
通讯作者：
H. Farach;D. Mundy;W. Strittmatter;W. Lennarz
无鞭毛海胆精子头中环磷酸腺苷浓度的升高。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Garbers,DL
通讯作者：
Garbers,DL
在精子中诱导顶体反应的岩藻糖-硫酸糖复合物会刺激肌醇 1,4,5-三磷酸积累。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Domino,SE;Garbers,DL
通讯作者：
Garbers,DL