Amphiphilic DNA tiles for controlled insertion and 2D assembly on fluid lipid membranes: the effect on mechanical properties
Amphiphilic DNA tiles for controlled insertion and 2D assembly on fluid lipid membranes: the effect on mechanical properties
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DOI：
10.1039/c6nr07084a
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发表时间：
2017-03-07
期刊：
影响因子：
6.7
通讯作者：
Contera, Sonia
中科院分区：
文献类型：
--
作者：
Dohno, Chikara;Makishi, Shingo;Contera, Sonia
未来的脂质膜相关的DNA纳米结构有望找到从合成生物学到纳米医学的应用。在这里，我们设计和合成的DNA瓦片，并修改它们与两亲性共价部分。由亲水性二甘醇（DEG）和疏水性十二烷基组成的dod-DEG基团被引入到磷酸盐主链上，以产生两亲性DNA链，随后将其引入到DNA瓦片的一面。以这种方式，瓦片变得能够通过在双层核心内部插入疏水基团而稳定地结合到脂质膜。功能化的瓦片在溶液中不聚集。我们的研究结果表明，这些两亲性DNA瓦片可以绑定和组装成2D晶格的凝胶和流体脂质双层。DNA结构与膜的结合取决于膜的脂质相、Mg 2+阳离子的浓度、对DNA的两亲性修饰的长度以及瓦片内修饰的密度。基于原子力显微镜的力谱被用来研究插入的DNA瓦片对脂质膜的机械性能的影响。结果表明，DNA瓦片的插入产生了约100%的DNA瓦片。双层穿透力增加20%。
Future lipid membrane-associated DNA nanostructures are expected to find applications ranging from synthetic biology to nanomedicine. Here we have designed and synthesized DNA tiles and modified them with amphiphilic covalent moieties. dod-DEG groups, which consist of a hydrophilic diethylene glycol (DEG) and a hydrophobic dodecyl group, are introduced at the phosphate backbone to create amphiphilic DNA strands which are subsequently introduced into one face of the DNA tiles. In this way the tile becomes able to stably bind to lipid membranes by insertion of the hydrophobic groups inside the bilayer core. The functionalized tiles do not aggregate in solution. Our results show that these amphiphilic DNA tiles can bind and assemble into 2D lattices on both gel and fluid lipid bilayers. The binding of the DNA structures to membranes is dependent on the lipid phase of the membrane, the concentration of Mg2+ cations, the length of the amphiphilic modifications to the DNA as well as on the density of the modifications within the tile. Atomic force microscopy-based force spectroscopy is used to investigate the effect of the inserted DNA tiles on the mechanical properties of the lipid membranes. The results indicate that the insertion of DNA tiles produces an approx. 20% increase of the bilayer breakthrough force.