Marinobacter nanhaiticus sp nov., polycyclic aromatic hydrocarbon-degrading bacterium isolated from the sediment of the South China Sea
Marinobacter nanhaiticus sp nov., polycyclic aromatic hydrocarbon-degrading bacterium isolated from the sediment of the South China Sea
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南海海杆菌 sp.
DOI：
10.1007/s10482-012-9830-z
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发表时间：
2013-03-01
影响因子：
2.6
通讯作者：
Zheng, Li
中科院分区：
文献类型：
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作者：
Gao, Wei;Cui, Zhisong;Zheng, Li
从南海沉积物中分离出一种革兰氏阴性、杆状、微嗜盐兼性厌氧细菌，命名为菌株D15-8W(T)。研究发现，生长最佳条件为 25 A 摄氏度、pH 7.0 至 8.0 以及 1-5 % (w/v) NaCl。观察到该菌株利用多种有机底物和多环芳烃作为唯一碳源。基因组DNA的G+C含量测定为58.7%。发现主要的呼吸醌是 Q-9。重要的脂肪酸被确定为C-16:0、C-16:1 omega 9c、C-18:1 omega 9c、C-12:0 和C-14:0 3OH。 16S rRNA基因序列分析表明，菌株D15-8W(T)属于Marinobacter属的系统发育簇，与Marinobacter segnicrescens CGMCC 1.6489(T)、Marinobacter bryozoorum DSM 15401(T)、Marinobacter lacisalsi CECT 7297(T)和Marinobacter daqiaonensis关系最密切。 CGMCC1.9167(T)。菌株D15-8W(T)与最密切相关物种的模式菌株之间的DNA-DNA杂交值分别为42.3%(CGMCC 1.6489(T))、39.8%(DSM 15401(T))、37.3%(CECT 7297(T))和35.2%(CGMCC1.9167(T))。这项多相研究的结果表明，菌株 D15-8W(T) 代表了海杆菌属的一个新物种，其名称为 Marinobacter nanhaiticus sp。十一月被提议。模式菌株是D15-8W(T) (=CGMCC 1.11019(T)=KCTC 23749(T))。
A Gram-negative, rod-shaped, slightly halophilic and facultatively anaerobic bacterium, designated strain D15-8W(T), was isolated from the sediment of the South China Sea. Growth was found to occur optimally at 25 A degrees C, between pH 7.0 and 8.0 and with 1-5 % (w/v) NaCl. The strain was observed to utilize a variety of organic substrates and polycyclic aromatic hydrocarbons as sole carbon sources. The G+C content of the genomic DNA was determined to be 58.7 %. The predominant respiratory quinone was found to be Q-9. The significant fatty acids were determined to be C-16:0, C-16:1 omega 9c, C-18:1 omega 9c, C-12:0 and C-14:0 3OH. Analysis of 16S rRNA gene sequences showed that strain D15-8W(T) fits within the phylogenetic cluster of the genus Marinobacter and is most closely related to Marinobacter segnicrescens CGMCC 1.6489(T), Marinobacter bryozoorum DSM 15401(T), Marinobacter lacisalsi CECT 7297(T) and Marinobacter daqiaonensis CGMCC1.9167(T). The DNA-DNA hybridization values between strain D15-8W(T) and the type strains of the most closely related species were 42.3 % (CGMCC 1.6489(T)), 39.8 % (DSM 15401(T)), 37.3 % (CECT 7297(T)) and 35.2 % (CGMCC1.9167(T)). The results of this polyphasic study indicate that strain D15-8W(T) represents a novel species of the genus Marinobacter, for which the name Marinobacter nanhaiticus sp. nov. is proposed. The type strain is D15-8W(T) (=CGMCC 1.11019(T)=KCTC 23749(T)).