Wound repair during arm regeneration in the red starfish Echinaster sepositus
Wound repair during arm regeneration in the red starfish Echinaster sepositus
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DOI：
10.1111/wrr.12333
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发表时间：
2015-07-01
影响因子：
2.9
通讯作者：
Sugni, Michela
中科院分区：
文献类型：
--
作者：
Ben Khadra, Yousra;Ferrario, Cinzia;Sugni, Michela
海星可以通过自体截肢和创伤性截肢来再生失去的整个手臂。尽管在不同的小行星物种中已经多次研究了整个再生过程，但关于组织和细胞层面修复阶段的详细方面，尤其是创伤后再生，仍然存在相当大的知识差距。本工作主要研究地中海红海星紫锥菊的手臂再生模型；为了描述创伤性截肢后手臂再生的早期细胞机制，采用了不同的显微镜技术。在脂溢性棘球绦虫中，修复阶段的特征是通过吞噬细胞的合体网络迅速愈合伤口，并重新上皮化，随后形成局部的表皮下水肿区。在再生的第一阶段，创面常可见散在的、明显未分化的细胞与大量吞噬细胞混合在一起，在残肢水平可见广泛的脱分化现象，尤其是在肌束中。未形成真正的局限性胚泡。我们的结果证实，小行星的再生主要依赖于变形过程，包括对现有组织的广泛重组，这些组织通过细胞去分化、再分化和/或迁移来促进新组织的形成。
Starfish can regenerate entire arms following their loss by both autotomic and traumatic amputation. Although the overall regenerative process has been studied several times in different asteroid species, there is still a considerable gap of knowledge as far as the detailed aspects of the repair phase at tissue and cellular level are concerned, particularly in post-traumatic regeneration. The present work is focused on the arm regeneration model in the Mediterranean red starfish Echinaster sepositus; to describe the early cellular mechanisms of arm regeneration following traumatic amputation, different microscopy techniques were employed. In E. sepositus, the repair phase was characterized by prompt wound healing by a syncytial network of phagocytes and re-epithelialisation followed by a localized subepidermal oedematous area formation. Scattered and apparently undifferentiated cells, intermixed with numerous phagocytes, were frequently found in the wound area during these first stages of regeneration and extensive dedifferentiation phenomena were seen at the level of the stump, particularly in the muscle bundles. A true localized blastema did not form. Our results confirm that regeneration in asteroids mainly relies on morphallactic processes, consisting in extensive rearrangement of the existing tissues which contribute to the new tissues through cell dedifferentiation, redifferentiation, and/or migration.