Reduction of Tomato-Plant Chilling Tolerance by CRISPR-Cas9-Mediated SICBF1 Mutagenesis
Reduction of Tomato-Plant Chilling Tolerance by CRISPR-Cas9-Mediated SICBF1 Mutagenesis
复制标题
通过 CRISPR-Cas9 介导的 SICBF1 诱变降低番茄植株的耐冷性
DOI：
10.1021/acs.jafc.8b02177
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发表时间：
2018-08-29
影响因子：
6.1
通讯作者：
Shen, Lin
中科院分区：
文献类型：
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作者：
Li, Rui;Zhang, Lixing;Shen, Lin
番茄是一种对低温敏感的园艺作物，低温胁迫是番茄生产的主要限制因素。高度保守的C-重复序列结合因子（CBF）是在许多物种中发现的冷反应系统的组成部分。在这项研究中，我们使用CRISPR-Cas9系统产生了slcbfl突变体，并研究了SICBF 1在番茄植物耐冷性中的作用。slcbfl突变体表现出更严重的冷害症状，更高的电解质渗漏和丙二醛水平比野生型（WT）植物。此外，slcbfl突变体表现出较低的脯氨酸和蛋白质含量和较高的过氧化氢含量和抗氧化酶的活性比野生型植物。SICBF 1基因敲除显著增加了吲哚乙酸的含量，但降低了茉莉酸甲酯、脱落酸和玉米素核苷的含量。slcbfl突变体的耐冷性降低进一步反映在CBF相关基因的下调上。这些结果有助于更好地了解SICBF 1介导番茄冷敏感性的分子基础。
Chilling stress is the main constraint in tomato (Solanum lycopersicum) production, as this is a chilling-sensitive horticultural crop. The highly conserved C-repeat binding factors (CBFs) are cold-response-system components found in many species. In this study, we generated slcbfl mutants using the CRISPR-Cas9 system and investigated the role of SICBF1 in tomato-plant chilling tolerances. The slcbfl mutants exhibited more severe chilling-injury symptoms with higher electrolyte leakage and malondialdehyde levels than wild-type (WT) plants. Additionally, slcbfl mutants showed lower proline and protein contents and higher hydrogen peroxide contents and activities of antioxidant enzymes than WT plants. Knockout of SICBF1 significantly increased indole acetic acid contents but decreased methyl jasmonate, abscisic acid, and zeatin riboside contents. The reduced chilling tolerance of the slcbfl mutants was further reflected by the down-regulation of CBF-related genes. These results contribute to a better understanding of the molecular basis underlying SICBF1 mediation of tomato chilling sensitivity.