IMMUNOHISTOCHEMICAL DEMONSTRATION OF VITRONECTIN IN ASSOCIATION WITH ELASTIN AND AMYLOID DEPOSITS IN HUMAN-KIDNEY
IMMUNOHISTOCHEMICAL DEMONSTRATION OF VITRONECTIN IN ASSOCIATION WITH ELASTIN AND AMYLOID DEPOSITS IN HUMAN-KIDNEY
复制标题
DOI：
10.1007/bf00492465
复制
发表时间：
1987-01-01
期刊：
影响因子：
--
通讯作者：
DAHLBACK, B
中科院分区：
文献类型：
--
作者：
DAHLBACK, K;LOFBERG, H;DAHLBACK, B
多功能糖蛋白Vitronectin，又称血清扩散因子和补体S蛋白，是体外细胞黏附和扩散的有力诱导剂，在补体和凝血途径中也具有调节作用。它存在于血浆和组织中。最近，在正常人皮肤的弹性纤维中发现了玻璃连蛋白的免疫反应。采用抗生物素-生物素-过氧化物酶复合体技术和碱性磷酸酶抗碱性磷酸酶复合体技术，用多克隆抗体和单抗研究了正常肾组织和淀粉样蛋白肾组织中玻璃体连结蛋白的免疫反应性。正常肾小球壁弹力层和良性肾硬化组肾小球硬化性病变中均可见Vitronectin的表达，而正常肾小球中未见Vit表达。在A型淀粉样变性和轻链型淀粉样变性患者肾脏的淀粉样蛋白沉积中均发现有较强的玻璃体连结蛋白免疫反应。抗淀粉样蛋白A抗血清免疫染色的结构与抗Vitronectin免疫染色的结构一致。抗血清淀粉样蛋白P组分的抗血清与抗玻璃体连接蛋白抗体染色的结构相同，此外还染色正常的肾小球基底膜。综上所述，在人肾的弹性组织、淀粉样蛋白沉积和硬化性病变中显示出玻璃体连结蛋白免疫反应。
The multifunctional glycoprotein vitronectin, also called serum spreading factor and S-protein of complement, is a potent inducer of cell adhesion and spreading in vitro, and also has a regulatory function in the complement and coagulation pathways. It is present both in plasma and tissue. Recently, vitronectin immunoreactivity was demonstrated in the elastic fibres of normal human skin. Normal and amyloid kidney tissue was investigated for vitronectin immunoreactivity using polyclonal and monoclonal antibodies in an avidin-biotin-peroxidase complex technique and in an alkaline phosphatase anti-alkaline phosphatase complex technique. Vitronectin was found in the elastic layers of normal vessel walls, and in glomerular sclerotic lesions in cases of benign nephrosclerosis, but not in normal glomeruli. Strong specific vitronectin immunoreactivity was found in the amyloid deposits in kidneys from cases with amyloid A type amyloidosis, and in cases with amyloid light chain type amyloidosis. Structures immunostainable with anti-amyloid A antiserum were invariably immunostainable with anti-vitronectin. An antiserum against serum amyloid P component stained the same structures as did the anti-vitronectin antibodies, and in addition stained normal glomerular basement membranes. In conclusion, vitronectin immunoreactivity was demonstrated in elastic tissue, in amyloid deposits and in sclerotic lesions in human kidney.