Super-Resolution Microscopy: Shedding Light on the Cellular Plasma Membrane.
Super-Resolution Microscopy: Shedding Light on the Cellular Plasma Membrane.
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DOI：
10.1021/acs.chemrev.6b00716
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发表时间：
2017-06-14
期刊：
影响因子：
62.1
通讯作者：
Veatch SL
中科院分区：
文献类型：
--
作者：
Stone MB;Shelby SA;Veatch SL
脂质及其形成的膜是细胞生命的基本组成部分，其几何形状和化学特性将膜与其他细胞环境区分开来。膜内发生的集体过程强烈影响细胞行为和生物化学，并且由于膜成分之间通常复杂且无数的相互作用，理解这些过程提出了独特的挑战。与传统光学显微镜相比，超分辨率显微镜的分辨率显着提高，即使在密集标记的样品以及细胞和组织环境中也能定位单个分子。这些显微镜技术已用于检查质膜成分的组织和动力学，从而深入了解决定膜功能的基本相互作用。在这里，我们广泛介绍哺乳动物质膜的结构和组织，并回顾超分辨率显微镜在膜研究中的最新应用。然后，我们强调使用超分辨率显微镜研究膜时面临的一些固有挑战，并讨论最近的技术进步，这些进步有望进一步改进超分辨率显微镜及其在质膜上的应用。
Lipids and the membranes they form are fundamental building blocks of cellular life, and their geometry and chemical properties distinguish membranes from other cellular environments. Collective processes occurring within membranes strongly impact cellular behavior and biochemistry, and understanding these processes presents unique challenges due to the often complex and myriad interactions between membrane components. Super-resolution microscopy offers a significant gain in resolution over traditional optical microscopy, enabling the localization of individual molecules even in densely labeled samples and in cellular and tissue environments. These microscopy techniques have been used to examine the organization and dynamics of plasma membrane components, providing insight into the fundamental interactions that determine membrane functions. Here, we broadly introduce the structure and organization of the mammalian plasma membrane and review recent applications of super-resolution microscopy to the study of membranes. We then highlight some inherent challenges faced when using super-resolution microscopy to study membranes, and we discuss recent technical advancements that promise further improvements to super-resolution microscopy and its application to the plasma membrane.