Cellular recognition of functionalized with folic acid nanoparticles
Cellular recognition of functionalized with folic acid nanoparticles
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DOI：
10.1380/ejssnt.2007.23
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发表时间：
2007-01
影响因子：
0.7
通讯作者：
S. Taira;T. Hatanaka;S. Moritake;Y. Kai;Y. Ichiyanagi;M. Setou
中科院分区：
文献类型：
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作者：
S. Taira;T. Hatanaka;S. Moritake;Y. Kai;Y. Ichiyanagi;M. Setou
我们已经制备了极小的功能化纳米颗粒（NPs），并表明它们可以在不进行修饰（例如阳离子涂层）的情况下引入活细胞中以增强内吞内化。然而，内化依赖于颗粒在细胞表面的物理吸附，因此内化过程是非选择性的。在这里，我们制备了与叶酸和香豆素荧光团缀合的NP，使得NP（d = 2.6 nm）将被细胞表面上的叶酸受体识别。通过傅里叶变换红外光谱法证实了纳米颗粒表面上叶酸和香豆素的存在。与不含叶酸的NP内化所需的时间相比，在较短的孵育时间后，经修饰的NP被人咽癌细胞（KB细胞）内化。该结果表明KB细胞膜上的叶酸受体识别叶酸缀合的NP。细胞识别的纳米颗粒可能会导致发展的细胞特异性输送系统。[DOI：10.1380/ejssnt.2007.23]
We have prepared extremely small functionalized nanoparticles (NPs) and showed that they could be introduced into living cells without modification (e.g. cationic coating) to enhance endocytic internalization. However, the internalization depended on physical adsorption of the particles on the cell surfaces, and therefore the internalization process was nonselective. Here, we prepared NPs conjugated with folic acid and a coumarin fluorophore so that the NPs (d = 2.6 nm) would be recognized by folate receptors on the cell surface. The presence of the folic acid and coumarin on the surface of the NPs was confirmed by Fourier transform infrared spectroscopy. The modified NPs were internalized by human pharyngeal cancer cells (KB cells) after an incubation time that was short compared with the time required for internalization of NPs without folic acid. This result indicates that the folic acid receptor on the KB cell membrane recognized the folic acid-conjugated NPs. Cellular recognition of NPs may lead to the development of cell-specific delivery systems. [DOI: 10.1380/ejssnt.2007.23]