IDENTIFICATION OF THE PROTEIN AND CDNA OF THE CARDIAC NA+/H+ EXCHANGER
IDENTIFICATION OF THE PROTEIN AND CDNA OF THE CARDIAC NA+/H+ EXCHANGER
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DOI：
10.1016/0014-5793(91)80241-t
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发表时间：
1991-02-11
期刊：
影响因子：
3.5
通讯作者：
BARR, A
中科院分区：
文献类型：
--
作者：
FLIEGEL, L;SARDET, C;BARR, A
我们检查了心肌形式的Na+/H+交换器。从兔心脏文库中分离到一个部分长度的cDNA克隆，它编码Na+/H+交换蛋白。与人Na ~+/H ~+交换蛋白相比，该cDNA的5'端序列高度保守，远高于3'端，推导的氨基酸序列也高度保守。为了进一步表征心肌Na+/H+交换蛋白，我们用针对Na+/H+交换器融合蛋白产生的抗体检测了分离的肌膜的Western印迹。抗体与50 kDa的肌膜蛋白和70 kDa的蛋白反应。结果表明，兔心肌中存在与人Na+/H+交换蛋白同源的Na+/H+交换蛋白。
We examined the myocardial form of the Na+/H+ exchanger. A partial length cDNA clone was isolated from a rabbit cardiac library and it encoded for a Na+/H+ exchange protein. In comparison with the human Na+/H+ exchanger, the sequence of the 5' end of the cDNA was highly conserved, much more than the 3' region, while the deduced amino acid sequence was also highly conserved. To further characterize the myocardial Na+/H+ exchange protein, we examined Western blots of isolated sarcolemma with antibody produced against a fusion protein of the Na+/H+ exchanger. The antibodies reacted with a sarcolemma protein of 50 kDa and with a protein of 70 kDa. The results show that the rabbit myocardium does possess a Na+/H+ exchanger protein homologous to the known human Na+/H+ exchanger.