Submicron Trenches Reduce the Pseudomonas fluorescens Colonization Rate on Solid Surfaces
Submicron Trenches Reduce the Pseudomonas fluorescens Colonization Rate on Solid Surfaces
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DOI：
10.1021/am8000677
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发表时间：
2009-01-01
影响因子：
9.5
通讯作者：
Fernandez Lorenzo de Mele, Monica A.
中科院分区：
文献类型：
--
作者：
Diaz, Carolina;Schilardi, Patricia L.;Fernandez Lorenzo de Mele, Monica A.
细菌在生物材料上的粘附和传播被认为是致病性的关键特征。据报道，粗糙度和地形的基板影响细菌的粘附，但很少有人知道他们的传播效果。设计了具有细菌直径（S2）的亚微米排和通道调谐以测试这些表面上的细菌运动性。随机纳米尺寸的结构（S1）被用作对照。光学显微镜和原子力显微镜分别用于检测微纳米级和纳米级的生物和表面图案细节。结果表明，运动策略（鞭毛方向，伸长。筏中的聚集、网络结构的形成和细菌边界的发展）受到亚微模式的存在的影响。重要的是，S2上的细菌传播速率显著降低，并受到亚微模式方向的影响。因此，亚微工程基质可以用作降低细菌定植的工具。这种模式可能会影响适当的工程结构的设计，以控制生物膜在固体表面上的传播。
Bacterial adhesion and spreading on biomaterials are considered key features of pathogenicity. Roughness and topography of the substrate have been reported to affect bacterial adhesion, but little is known about their effect on spreading. Submicron row and channel tuning with bacterial diameter (S2) were designed to test bacterial motility on these surfaces. Random nanometer-sized structures (S1) were used as controls. Optical microscopy and AFM were employed to detect biological and surface pattern details in the micro- and nanoscale, respectively. Results showed that motility strategies (flagella orientation, elongation. aggregation in rafts, formation of network structures, and development of a bacterial frontier) were affected by the presence of submicropatterns. Importantly, the rate of bacterial spreading on S2 was significantly reduced and influenced by the orientation of the submicropatterns. Consequently, submicroengineered substrates could be employed as a tool to downgrade bacterial colonization. Such patterns could impact on the design of proper engineered structures to control biofilm spreading on solid surfaces.