Multigene editing via CRISPR/Cas9 guided by a single-sgRNA seed in Arabidopsis.
Multigene editing via CRISPR/Cas9 guided by a single-sgRNA seed in Arabidopsis.
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DOI：
10.1111/jipb.12622
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发表时间：
2018-05
影响因子：
11.4
通讯作者：
Zhiming Yu;Qiyuan Chen;Weiwei Chen;Xian Zhang;Fengling Mei;Pengcheng Zhang;Mei Zhao;Xiaohong Wang;N. Shi;S. Jackson;Yiguo Hong
中科院分区：
文献类型：
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作者：
Zhiming Yu;Qiyuan Chen;Weiwei Chen;Xian Zhang;Fengling Mei;Pengcheng Zhang;Mei Zhao;Xiaohong Wang;N. Shi;S. Jackson;Yiguo Hong
我们报道了一个单独的单向导RNA（sgRNA）种子能够引导重复的规则间隔短回文重复序列（CRISPR）/CRISPR相关9（CRISRP/Cas9）在拟南芥中同时编辑多个基因AtRPL 10A、AtRPL 10 B和AtRPL 10 C。我们的研究结果还表明，可以使用CRISPR/Cas9技术来创建AtRPL 10三重突变体，否则无法通过常规遗传杂交产生。与其他传统的多重CRISPR/Cas系统相比，单个sgRNA种子具有减少脱靶基因编辑的优势。这样的基因编辑系统也可以适用于修饰其他同源基因，或者甚至用于植物和其他生物体中的多个基因编辑的不太同源的序列。
We report that a solo single-guide RNA (sgRNA) seed is capable of guiding Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR -associated 9 (CRISRP/Cas9) to simultaneously edit multiple genes AtRPL10A, AtRPL10B and AtRPL10C in Arabidopsis. Our results also demonstrate that it is possible to use CRISPR/Cas9 technology to create AtRPL10 triple mutants which otherwise cannot be generated by conventional genetic crossing. Compared to other conventional multiplex CRISPR/Cas systems, a single sgRNA seed has the advantage of reducing off-target gene-editing. Such a gene editing system might be also applicable to modify other homologous genes, or even less-homologous sequences for multiple gene-editing in plants and other organisms.