Electrophysiological analysis of the mechanism of autonomic action by lactobacilli.
Electrophysiological analysis of the mechanism of autonomic action by lactobacilli.
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DOI：
10.12938/bifidus.30.99
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Nagai, Katsuya
中科院分区：
文献类型：
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作者：
Tanida, Mamoru;Nagai, Katsuya
自主神经包括交感神经和副交感神经，调节各种身体功能，如血压、体温、葡萄糖代谢、能量代谢和消化。我们在大鼠和小鼠身上的研究表明，食物、味道和音乐通过改变自主神经传递来影响生理现象。肠道注射约翰乳杆菌La1(NCC533)抑制了支配乌拉坦麻醉大鼠肾上腺和肾脏的交感神经，降低了血糖和血压水平，兴奋了胃副交感神经，提高了食欲和体重。相比之下，肠道注射副乳杆菌ST11(NCC2461)兴奋了支配白色和棕色脂肪和肾上腺的交感神经，增加了脂肪分解和体温，抑制了胃副交感神经，减少了食欲和体重。有趣的是，我们发现作为生物钟的下丘脑视交叉上核(SCN)和组胺能神经元中的组胺受体在外周自主神经控制中起着重要作用。为了研究SCN和组胺受体在乳杆菌介导的病理中的可能作用，我们建立了SCN损伤模型，并实验了组胺能阻滞剂注射。损毁SCN或注射组胺H3受体拮抗剂硫代巴比妥钠可消除NCC533对肾交感神经活动的抑制，防止血压下降，并抑制NCC533引起的胃副交感神经的增强。此外，组胺H1受体拮抗剂苯海拉明可阻断NCC2461引起的肾交感神经活动和血压的增加。隔下迷走神经切断术可消除NCC533对肾交感神经活动的抑制，但不影响NCC2461对肾交感神经的兴奋作用。总之，这些发现强烈表明SCN和组胺神经元通过腹部传入迷走神经通路进入中枢参与了乳酸杆菌介导的自主神经的病理和相关的生理变化。
Autonomic nerves, consisting of both sympathetic and parasympathetic nerves, regulate various bodily functions such as blood pressure, body temperature, glucose metabolism, energy metabolism, and digestion. Our studies in rats and mice have demonstrated that food, flavor, and music affect physiological phenomena via changes in autonomic neurotransmissions. Intestinal injection of Lactobacillus johnsonii La1 (NCC533) suppressed sympathetic nerves that innervate the adrenal gland and kidney of urethane-anesthetized rats, lowering blood glucose and blood pressure levels, and excited the gastric parasympathetic nerve, elevating appetite and body weight. In contrast, intestinal injection of Lactobacillus paracasei ST11 (NCC2461) excited sympathetic nerves that innervate white and brown fat and the adrenal gland, increasing lipolysis and body temperature, and suppressed the gastric parasympathetic nerve, reducing appetite and body weight. Interestingly, we found that the hypothalamic suprachiasmatic nucleus (SCN), a master circadian clock, and histamine receptors in histaminergic neurons play important roles in peripheral autonomic control. To investigate the possible role of SCN and histamine receptors in lactobacilli-mediated pathology, we created an SCN-lesion model and experimented with histaminergic blocker injections. SCN lesion or injection of thioperamide, a histamine H3-receptor antagonist, eliminated the suppression of renal sympathetic nerve activity by NCC533, preventing blood pressure decline, and inhibited the enhancement of the gastric parasympathetic nerve induced by NCC533. In addition, diphenhydramine, a histamine H1-receptor antagonist, abolished the increases in renal sympathetic nerve activity and blood pressure caused by NCC2461. Infradiaphragmatic vagotomy eliminated the suppression of renal sympathetic nerve activity by NCC533, but did not affect the excitation of the renal sympathetic nerve by NCC2461. Collectively, these findings strongly suggest that SCN and histamine neurons are involved in the lactobacilli-mediated pathology of autonomic nerves and related physiological changes through abdominal afferent vagal pathway input to the central nervous system.