Delimiting a rice wide-compatibility gene S5n supercript stop to a 50 kb region
Delimiting a rice wide-compatibility gene S5n supercript stop to a 50 kb region
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DOI：
10.1007/s00122-005-0078-0
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发表时间：
2005-11-01
影响因子：
5.4
通讯作者：
Xu, ML
中科院分区：
文献类型：
--
作者：
Ji, Q;Lu, JF;Xu, ML
广亲和性是水稻的重要性状之一，它能克服籼粳杂种的育性障碍，使亚种间杂种的高产潜力得以利用。位于水稻第6染色体上的S-5（n）基因是水稻广亲和的主要基因。在这里，我们报告的S-5（n）基因的精确定位。在第一遍定位中，通过使用242个等基因系的群体结合在S-5区域中开发的高密度标记将S-5（n）基因限制在200 kb区域内。在精细定位中，S-5区域进一步被新开发的标记饱和，并调查了更多的等基因系（共549个）。最终，S-5（n）基因定位在由左标记J13和右标记J17限定的50 kb区域内。从WC水稻品种02428的BAC文库中筛选到一个BAC克隆，覆盖了整个S-5区。50 kb区域的序列分析揭示了两个候选基因，编码一个乙酰基蛋白酶和一个假设的蛋白质。这一结果将大大加快这一重要的S-5（n）基因的克隆和标记辅助选择。
Wide-compatibility (WC) is one of the most important traits in rice, which can overcome the fertility barrier in the indica/japonica hybrids, and hence to make it possible to utilize the higher yield potential of inter-subspecific hybrids. The S-5(n) gene located on chromosome 6 has been previously reported to be responsible for the wide-compatibility in rice. Here we report the precise location of the S-5(n) gene. In the first-pass mapping, the S-5(n) gene was restricted within a 200 kb region by using a population of 242 isogenic lines in combination with high-density markers developed in the S-5 region. In the fine mapping, the S-5 region was further saturated with newly developed markers and more isogenic lines (549 in total) were investigated. Eventually, the S-5(n) gene was mapped within a 50 kb region delimited by the left marker J13 and the right marker J17. One BAC clone screened from the BAC library of the WC rice variety 02428 covered the whole S-5 region. Sequence analysis of the 50 kb region revealed two candidate genes, coding an aspartyl protease and a hypothetical protein. This result would greatly accelerate both cloning and marker-assisted selection of this important S-5(n) gene.