GENETIC-VARIATION WITHIN A NEUTRALIZING DOMAIN ON THE HEMAGGLUTININ NEURAMINIDASE GLYCOPROTEIN OF NEWCASTLE-DISEASE VIRUS
GENETIC-VARIATION WITHIN A NEUTRALIZING DOMAIN ON THE HEMAGGLUTININ NEURAMINIDASE GLYCOPROTEIN OF NEWCASTLE-DISEASE VIRUS
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DOI：
10.1099/0022-1317-67-7-1393
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发表时间：
1986-07-01
影响因子：
3.8
通讯作者：
BRATT, MA
中科院分区：
文献类型：
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作者：
IORIO, RM;BORGMAN, JB;BRATT, MA
以前，一组识别纽卡斯尔病病毒澳大利亚-维多利亚株血凝素-神经氨酸酶（HN）糖蛋白上四个抗原位点表位的单克隆抗体用于株系比较。发现三个位点中的表位是保守的，而位点3的单抗识别的表位不是保守的。描述了一组新的抗体，其中两个结合位点3中的表位，其中六个结合位点（位点1，4），所述位点与位点1和4重叠，如通过在放射免疫测定中通过中和感染性和结合的测定分析变体、温度敏感突变体和菌株所确定的。用一组抗体中和异源菌株显示，两个新的位点3表位也是高度不同的，而位点3外的三个另外的表位（位点1、4中的那些）是高度保守的。新的位点3抗体可以结合几种异源菌株的病毒粒子而不中和感染性。因此，在我们现在检查的10个表位中，位点3中的所有3个表位对于同源菌株的感染性中和是特异性的，而其他位点中的所有7个表位在异源菌株中是保守的。这表明最初针对单个位点3表位描述的菌株特异性实际上是HN分子上更广泛、保守性较差的结构域的特性。
Previously, a panel of monoclonal antibodies recognizing epitopes in four antigenic sites on the haemagglutinin-neuraminidase (HN) glycoprotein of the Australia-Victoria strain of Newcastle disease virus were used in strain comparisons. Epitopes in three sites were found to be conserved while the epitope recognized by the single antibody to site 3 was not. A new panel of antibodies is described, two of which bind to epitopes in site 3 and six of which bind to a site (site 1,4) that overlaps with sites 1 and 4 as determined by analyses of variants, temperature-sensitive mutants, and strains by assays of neutralization of infectivity and binding in a radioimmunoassay. Neutralization of heterologous strains with the panel of antibodies revealed that both new site 3 epitopes are also highly divergent, while three additional epitopes outside site 3 (those in site 1,4) are highly conserved. The new site 3 antibodies can bind to virions of several heterologous strains without neutralizing infectivity. Thus, of the 10 epitopes we have now examined, all of three in site 3 are specific with respect to neutralization of infectivity for the homologous strain, while all of seven in other sites are conserved in heterologous strains. This suggests that the strain specificity originally described for a single site 3 epitope is, instead, a property of a much more extensive, poorly conserved domain on the HN molecule.