Molecular analysis of human forearm superficial skin bacterial biota
Molecular analysis of human forearm superficial skin bacterial biota
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DOI：
10.1073/pnas.0607077104
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发表时间：
2007-02-20
影响因子：
11.1
通讯作者：
Blaser, Martin J.
中科院分区：
文献类型：
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作者：
Gao, Zhan;Tseng, Chi-hong;Blaser, Martin J.
人类皮肤的微生物生态是复杂的，但对其物种组成知之甚少。我们研究了6名健康受试者掌侧、左侧和右侧前臂浅层皮肤生物群的多样性，方法是对随机选择的克隆进行基于广谱小亚基rRNA基因(16S RDNA)的测序。在所分析的最初的1221个无性系中，共鉴定出182个种水平的可操作分类单元(SLOTU)，隶属于8个门，估计占该生态系统中SLOTU的74.0%[95%可信区间(C.I.)，约为64.8-77.9%]，每个对象中有48.0+/-12.2个SLOTU。3门(放线杆菌、菲尔米特和变形杆菌)占克隆总数的94.6%。大多数细菌序列(85.3%)与已知和栽培种相对应，但98个克隆(8.0%)与先前的数据库序列有97%的相似性，包括30个系统发育型。91个属中只有6个(6.6%)，182个SLOTU中只有4个(2.2%)。对8-10个月后从4个受试者获得的817个克隆的分析显示，额外的门(编号2)、属(编号28)和SLOTU(编号65)。在两次测试的119个属中，只有4个属(丙酸杆菌、棒状杆菌、葡萄球菌和链球菌)被观察到，但这些属占所有克隆的54.4%。这些结果表明，正常浅表皮肤中的细菌生物群高度多样化，几乎没有保存良好和有代表性的属，但除此之外，人际间的共识很低。
The microbial ecology of human skin is complex, but little is known about its species composition. We examined the diversity of the skin biota from the superficial volar left and right forearms in six healthy subjects using broad-range small subunit rRNA genes (16S rDNA) PCR-based sequencing of randomly selected clones. For the initial 1,221 clones analyzed, 182 species-level operational taxonomic units (SLOTUs) belonging to eight phyla were identified, estimated as 74.0% [95% confidence interval (C.I.), approximate to 64.8-77.9%] of the SLOTUs in this ecosystem; 48.0 +/- 12.2 SLOTUs were found in each subject. Three phyla (Actinobacteria, Firmicutes, and Proteobacteria) accounted for 94.6% of the clones. Most (85.3%) of the bacterial sequences corresponded to known and cultivated species, but 98 (8.0%) clones, comprising 30 phylotypes, had < 97% similarity to prior database sequences. Only 6 (6.6%) of the 91 genera and 4 (2.2%) of the 182 SLOTUs, respectively, were found in all six subjects. Analysis of 817 clones obtained 8-10 months later from four subjects showed additional phyla (numbering 2), genera (numbering 28), and SLOTUs (numbering 65). Only four (3.4%) of the 119 genera (Propionibacteria, Corynebacteria, Staphylococcus, and Streptococcus) were observed in each subject tested twice, but these genera represented 54.4% of all clones. These results show that the bacterial biota in normal superficial skin is highly diverse, with few well conserved and well represented genera, but otherwise low-level interpersonal consensus.