Differential patterns of epidermal leukocyte infiltration in patch test reactions to structurally unrelated chemical irritants.
Differential patterns of epidermal leukocyte infiltration in patch test reactions to structurally unrelated chemical irritants.
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对结构无关的化学刺激物的斑贴试验反应中表皮白细胞浸润的差异模式。
DOI：
10.1111/1523-1747.ep12365561
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
J. Wilkinson
中科院分区：
文献类型：
--
作者：
C. Willis;C. Stephens;J. Wilkinson
在以前的研究中，我们发现刺激性接触性皮炎的组织病理学的许多方面受到所用刺激物的化学性质的深刻影响。我们在这里报告，这种现象也延伸到白细胞浸润到表皮。健康志愿者使用以下刺激物及其适当对照品进行斑贴试验：苯扎氯铵、十二烷基硫酸钠、巴豆油、地蒽酚、壬酸和丙二醇。在视觉上对所产生的炎症的强度进行分级后，取出活检组织，并通过免疫细胞化学方法鉴定白细胞的主要表型类别。真皮和表皮细胞密度进行了测定，并研究了几种活化/增殖抗原的表达。我们在所有刺激组的真皮中发现了类似的细胞浸润模式;大多数细胞类型的密度随着炎症的强度而升高。然而，在表皮内，刺激组之间的细胞浸润模式存在显著差异，导致白细胞密度和视觉分级之间的相关性较差。最大的差异发生在巴豆油和壬酸活检，前者的特点是大量白细胞的流入，后者表现出显着很少的胞吐作用。中性粒细胞浸润发生在不同程度上与所有刺激物，但不成比例的大量存在于十二烷基硫酸钠活检。所有对照组均显示CD 4+细胞增加，蒸馏水也导致CD 11c+细胞和中性粒细胞增加。刺激组和对照组的表皮中均出现了选择性的CD25+细胞流入。我们的观察进一步强调了刺激性接触性皮炎的异质性，并证实了以前的研究结果，即视觉阴性对照斑贴试验显示出明显的细胞反应性。
In previous studies, we showed that a number of aspects of the histopathology of irritant contact dermatitis are profoundly influenced by the chemical nature of the irritant applied. We report here that this phenomenon also extends to the infiltration of leukocytes into the epidermis. Healthy volunteers were patch tested with the following irritants and their appropriate controls: benzalkonium chloride, sodium lauryl sulphate, croton oil, dithranol, nonanoic acid, and propylene glycol. After visually grading the intensity of the resulting inflammation, biopsies were removed and the major phenotypic classes of leukocytes identified immunocytochemically. Dermal and epidermal cell densities were determined, and the expression of several activation/proliferation antigens studied. We found a similar pattern of cellular infiltration in the dermis of all irritant groups; the densities of most of the cell types rising in line with the intensity of inflammation. Within the epidermis, however, there were marked differences in the patterns of cellular infiltration between the irritant groups, leading to poorer correlations between leukocyte density and visual grading. The greatest disparity occurred between croton oil and nonanoic acid biopsies, the former being characterized by the influx of large numbers of leukocytes, the latter showing remarkably little exocytosis. Infiltration of neutrophils occurred to varying degrees with all irritants, but a disproportionately large number were present in sodium lauryl sulphate biopsies. All control groups showed a rise in CD4+ cells, with distilled water also producing increases in CD11c+ cells and neutrophils. A selective influx of CD25+ cells occurred in the epidermis of both irritant and control groups. Our observations further highlight the heterogeneous nature of irritant contact dermatitis, and confirm previous findings that visually negative control patch tests show marked cellular reactivity.
佛波醇肉豆蔻酸酯乙酸酯：体内对中性粒细胞、血小板和肺的影响。
DOI：
--
发表时间：
1980
期刊：
The American journal of pathology
影响因子：
--
作者：
O'Flaherty,JT;Cousart,S;Lineberger,AS;Bond,E;Bass,DA;DeChatelet,LR;Leake,ES;McCall,CE
通讯作者：
McCall,CE