Endothelial cell transdifferentiation of human glioma stem progenitor cells in vitro
Endothelial cell transdifferentiation of human glioma stem progenitor cells in vitro
复制标题
人胶质瘤干祖细胞的内皮细胞体外转分化。
DOI：
10.1016/j.brainresbull.2010.06.006
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发表时间：
2010-07-30
影响因子：
3.8
通讯作者：
Lan, Qing
中科院分区：
文献类型：
--
作者：
Zhao, Yaodong;Dong, Jun;Lan, Qing
正常干细胞在多种组织或器官中的转分化已被研究。然而，胶质瘤干细胞是否具有转分化能力的研究报道较少。与此同时，肿瘤血管生成的机制仍存在争议，胶质瘤干/祖细胞（GSPCs）是否参与胶质瘤血管生成尚不清楚。在本研究中，我们在内皮分化培养基中培养GSPCs 10天，发现它们呈现出典型的“石板”状的血管内皮细胞（VEC）的外观;当在Matrigel上培养时，GSPCs逐渐形成管状结构，并且形成管状结构的细胞在透射电镜下显示出与VEC相似的超微结构特征。在缺氧或缺糖缺氧条件下培养4 h，GSPC可转录表达VEC的分子标志物CD 31、CD 34和vWF。这些结果表明，GSPC可以通过转分化为VEC样细胞参与胶质瘤血管生成。(C)2010年爱思唯尔公司All rights reserved.
The transdifferentiation of normal stem cells in many kinds of tissues or organs has been studied. However, whether glioma stem cells can transdifferentiate is seldom reported. Meanwhile, the mechanism of angiogenesis in tumors is in disputations, and it is still unknown that whether glioma stem/progenitor cells (GSPCs) participate into angiogenesis in glioma. In this study, we cultivated GSPCs in endothelial differentiation medium for 10 days and found they present to be the typical "flagstone" appearance of vascular endothelial cell (VEC); when cultured on Matrigel, GSPCs gradually formed tubular-like structures in vitro, and cells, which formed the tubular-like structures, showed similar ultrastructural characteristics of VEC under a transmission electron microscope. Furthermore, when cultured in hypoxia or oxygen-glucose deprivation for 4 h, GSPCs transcribed and expressed molecular markers of VEC, including CD31, CD34 and vWF. These results indicated that GSPCs could participate into angiogenesis of glioma by transdifferentiating into VEC-like cells. (C) 2010 Elsevier Inc. All rights reserved.