Landscape of adenosine-to-inosine RNA recoding across human tissues.
Landscape of adenosine-to-inosine RNA recoding across human tissues.
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DOI：
10.1038/s41467-022-28841-4
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发表时间：
2022-03-04
影响因子：
16.6
通讯作者：
Eisenberg E
中科院分区：
文献类型：
--
作者：
Gabay O;Shoshan Y;Kopel E;Ben-Zvi U;Mann TD;Bressler N;Cohen-Fultheim R;Schaffer AA;Roth SH;Tzur Z;Levanon EY;Eisenberg E
通过腺苷脱氨酶进行RNA编辑可以改变mRNA基因组蓝图中编码的信息。编辑蛋白质编码序列可以引入新的、功能独特的蛋白质亚型，使蛋白质组多样化。几十年来，人们一直认识到一些重新编码位点的功能重要性。然而，揭示这些位点的系统方法表现不佳，而且人类和其他哺乳动物的全部重编码曲目尚不清楚。在这里，我们提出了一种新的检测方法，并分析了9125个GTEx RNA-seq样本，以产生编码区域内1517个编辑位点及其在人体组织中的编辑水平的高精度图谱。单细胞RNA-seq数据显示，蛋白质重编码有助于跨细胞亚群的变异性。大多数高度编辑的位点在非灵长类哺乳动物中是进化保守的，证明了适应性。这一综合集有助于理解重编码在人类生理和疾病中的作用。Gabay等人通过a -to- i RNA编辑在人类中提供了一个高度精确的重新编码图谱，并在组织和细胞亚群中进行了分析。大多数高度编辑的位点在非灵长类哺乳动物中是进化保守的，证明了适应性。
RNA editing by adenosine deaminases changes the information encoded in the mRNA from its genomic blueprint. Editing of protein-coding sequences can introduce novel, functionally distinct, protein isoforms and diversify the proteome. The functional importance of a few recoding sites has been appreciated for decades. However, systematic methods to uncover these sites perform poorly, and the full repertoire of recoding in human and other mammals is unknown. Here we present a new detection approach, and analyze 9125 GTEx RNA-seq samples, to produce a highly-accurate atlas of 1517 editing sites within the coding region and their editing levels across human tissues. Single-cell RNA-seq data shows protein recoding contributes to the variability across cell subpopulations. Most highly edited sites are evolutionary conserved in non-primate mammals, attesting for adaptation. This comprehensive set can facilitate understanding of the role of recoding in human physiology and diseases. Gabay et al. provide a highly-accurate atlas of recoding by A-to-I RNA editing in human, profiled across tissues and cell subpopulations. Most highly edited sites are evolutionary conserved in non-primate mammals, attesting for adaptation.
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DOI：
10.1261/rna.078804.121
发表时间：
2021-12
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Ansell BRE;Thomas SN;Bonelli R;Munro JE;Freytag S;Bahlo M
通讯作者：
Bahlo M
影响因子：
5.9
作者：
Brooks, Matthew J.;Chen, Holly Y.;Swaroop, Anand
通讯作者：
Swaroop, Anand
影响因子：
16.6
作者：
Eggington, Julie M.;Greene, Tom;Bass, Brenda L.
通讯作者：
Bass, Brenda L.
通过纯化选择塑造恒河猴的RNA编辑组
影响因子：
4.5
作者：
Chen JY;Peng Z;Zhang R;Yang XZ;Tan BC;Fang H;Liu CJ;Shi M;Ye ZQ;Zhang YE;Deng M;Zhang X;Li CY
通讯作者：
Li CY
影响因子：
4.4
作者：
D'Erchia, Anna Maria;Atlante, Anna;Pesole, Graziano
通讯作者：
Pesole, Graziano