Encapsulation of chondrocytes in injectable alkali-treated collagen gels prepared using poly(ethylene glycol)-based 4-armed star polymer
Encapsulation of chondrocytes in injectable alkali-treated collagen gels prepared using poly(ethylene glycol)-based 4-armed star polymer
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DOI：
10.1016/j.biomaterials.2004.04.029
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发表时间：
2005-04-01
期刊：
影响因子：
14
通讯作者：
Tanaka, J
中科院分区：
文献类型：
--
作者：
Taguchi, T;Xu, LM;Tanaka, J
以碱处理的胶原蛋白(AlCol)和季戊四醇聚乙二醇醚戊二酸丁二酰亚胺(4S-PEG)为交联剂，建立了一种生理条件下软骨细胞的原位凝胶体系。当交联剂浓度为0.1~3.0 mM时，可得到AlCol凝胶。软骨细胞被包裹并均匀分散在AlCol凝胶中。四甲基偶氮唑盐染色结果显示包埋在AlCol凝胶中的细胞存活。生化分析表明，AlCol凝胶中的DNA含量在3周后保持不变。随着培养时间的延长，11型胶原和聚集素的糖胺多糖含量和mRNA表达增加。这些结果表明，这种原位凝胶系统在体外再生软骨和微创治疗软骨缺损方面是有用的。(C)2004爱思唯尔有限公司。保留所有权利。
An in situ gel system was developed to encapsulate chondrocytes under physiological conditions using an alkali-treated collagen (AlCol) and pentaerythritol poly(ethylene glycol) ether tetrasuccinimidyl glutarate (4S-PEG) as a crosslinker. AlCol gels were obtained at crosslinker concentrations from 0.1 to 3.0 mm. Chondrocytes were encapsulated and dispersed homogeneously in AlCol gels. Results of MTT staining showed that cells survived after encapsulation in AlCol gels. Biochemical analysis demonstrated that DNA content in AlCol gels was constant after 3 weeks. Glycosaminoglycan content and mRNA expression of type 11 collagen and aggrecan increased with culture time. These results suggest that this in situ gel system is useful for regenerating cartilage in vitro and for minimally invasive therapy for cartilage defects. (C) 2004 Elsevier Ltd. All rights reserved.