Design, synthesis, and enzymatic property of a sulfur-substituted analogue of trigalacturonic acid
Design, synthesis, and enzymatic property of a sulfur-substituted analogue of trigalacturonic acid
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DOI：
10.1016/j.bmcl.2005.08.019
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发表时间：
2005-11-15
影响因子：
2.7
通讯作者：
Okuno, T
中科院分区：
文献类型：
--
作者：
Yamamoto, K;Watanabe, N;Okuno, T
合成了三半乳糖醛酸（3）的硫取代类似物。该合成的特点是3-氰基-3-（叔丁基二甲基硅基）氧丙基硫醚（CSP）作为一种新的保护基团的硫醇的应用。这种类似物的设计与期望，它将是一个稳定的类似物的基础上从革菌分离的内切多聚半乳糖醛酸酶的计算机模拟实验。表面等离子体共振实验表明，3与靶酶形成稳定的复合物。(c)2005爱思唯尔有限公司保留所有权利。
A sulfur-substituted analogue of trigalacturonic acid (3) was synthesized. The synthesis features the application of 3-cyano-3-(tert-butyldimethylsilyl)oxypropylthioether (CSP) as a novel protective group for thiols. This analogue was designed with the expectation that it would be a stable analogous substrate for endo-polygalacturonase isolated from Stereum purpureum based on computer modeling experiments. Surface plasmon resonance experiments revealed that 3 forms a stable complex with the target enzyme. (c) 2005 Elsevier Ltd. All rights reserved.