miR172 signals are incorporated into the miR156 signaling pathway at the SPL3/4/5 genes in Arabidopsis developmental transitions
miR172 signals are incorporated into the miR156 signaling pathway at the SPL3/4/5 genes in Arabidopsis developmental transitions
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DOI：
10.1007/s11103-011-9759-z
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发表时间：
2011-05-01
影响因子：
5.1
通讯作者：
Park, Chung-Mo
中科院分区：
文献类型：
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作者：
Jung, Jae-Hoon;Seo, Pil Joon;Park, Chung-Mo
在植物中，发育时间由整合了多种内在和外在信号的复杂信号网络协调调节。 miR172 促进光周期开花。它还与 miR156 一起调节成人发育，尽管这种调节背后的分子机制尚不完全清楚。在这里，我们证明 miR172 通过调节 SQUAMOSA 启动子结合蛋白样 (SPL) 基因子集的表达来调节发育转变，这些基因也受到 miR156 的调节。 SPL3/4/5 基因在 miR172 过量产生的植物 (35S:172) 及其表现出早期开花的靶基因突变体中上调。相比之下，其他 SPL 基因的表达没有改变到可辨别的水平。对表达由β-雌二醇诱导型启动子驱动的MIR156a基因的转基因植物中miR172丰度的动力学测量表明，miR172和miR156的表达并不直接相关。相反，2 个 miRNA 信号整合在 SPL3/4/5 基因上。值得注意的是，对过量产生 miR172 和 miR156 的 156 x 172 植物的发育模式分析表明，虽然在过量产生 miR156 的植物 (35S:156) 中观察到营养阶段变化被延迟，但如在 35S:172 转基因植物中观察到的开花起始加速。总之，这些观察结果表明，尽管 miR172 和 miR156 在发育阶段转变的时间中发挥不同的作用，但存在由 SPL3/4/5 基因介导的信号串扰。
In plants, developmental timing is coordinately regulated by a complex signaling network that integrates diverse intrinsic and extrinsic signals. miR172 promotes photoperiodic flowering. It also regulates adult development along with miR156, although the molecular mechanisms underlying this regulation are not fully understood. Here, we demonstrate that miR172 modulates the developmental transitions by regulating the expression of a subset of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes, which are also regulated by miR156. The SPL3/4/5 genes were upregulated in the miR172-overproducing plants (35S:172) and its target gene mutants that exhibit early flowering. In contrast, expression of other SPL genes was not altered to a discernible level. Kinetic measurements of miR172 abundance in the transgenic plants expressing the MIR156a gene driven by a beta-estradiol-inducible promoter revealed that expressions of miR172 and miR156 are not directly interrelated. Instead, the 2 miRNA signals are integrated at the SPL3/4/5 genes. Notably, analysis of developmental patterns in the 156 x 172 plants overproducing both miR172 and miR156 showed that whereas vegetative phase change was delayed as observed in the miR156-overproducing plants (35S:156), flowering initiation was accelerated as observed in the 35S:172 transgenic plants. Together, these observations indicate that although miR172 and miR156 play distinct roles in the timing of developmental phase transitions, there is a signaling crosstalk mediated by the SPL3/4/5 genes.