Deep sequencing of dsRNAs recovered from mosaic-diseased pigeonpea reveals the presence of a novel emaravirus: pigeonpea sterility mosaic virus 2
Deep sequencing of dsRNAs recovered from mosaic-diseased pigeonpea reveals the presence of a novel emaravirus: pigeonpea sterility mosaic virus 2
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DOI：
10.1007/s00705-015-2479-y
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发表时间：
2015-08-01
影响因子：
2.7
通讯作者：
Sudini, Harikishan
中科院分区：
文献类型：
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作者：
Elbeaino, Toufic;Digiaro, Michele;Sudini, Harikishan
对一株花叶病鸽子豌豆（Cajanus cajan L.， Fabaceae科）双链RNA进行深度测序分析，发现6个emarvirus样负义RNA片段的完整序列，大小分别为7009、2229、1335、1491、1833和1194个核苷酸。按照RNA1到RNA6的顺序，这些基因组RNA含有编码RNA依赖RNA聚合酶（RdRp， p1为266 kDa）、糖蛋白前体（GP， p2为74.5 kDa）、核衣壳（NC， p3为34.9 kDa）和推测的运动蛋白（MP， p4为40.7 kDa）的orf，而p5 （55 kDa）和p6 （27 kDa）的orf功能未知。所有RNA片段与Emaravirus属病毒，特别是鸽豌豆不育花叶病毒（PPSMV）的亲缘关系较远，核苷酸序列同源性从48.5% （RNA3）到62.5% （RNA1）不等。在由RNA1、RNA2和RNA3编码的蛋白序列（p1、p2和p3）构建的系统发育树中，该新病毒实体与无花果花叶病毒（FMV）和玫瑰花环病毒（RRV）形成了一个簇，与PPSMV-1明显不同。在温室试验中，这种新型病毒成功地通过叶面螨（Aceria cajani）传播到鸽豌豆和法国豆苗上。在海德拉巴地区（印度）进行的初步调查表明，该病毒在受不育花叶病影响的鸽子豌豆植物中广泛存在（86.4%），但在无症状植物中不存在。基于分子生物学和流行病学特征，该病毒是第二种感染鸽豌豆的emaravirus，暂定名为鸽豌豆不育花叶病毒2号（PPSMV-2）。
Deep-sequencing analysis of double-stranded RNA extracted from a mosaic-diseased pigeonpea plant (Cajanus cajan L., family Fabaceae) revealed the complete sequence of six emaravirus-like negative-sense RNA segments of 7009, 2229, 1335, 1491, 1833 and 1194 nucleotides in size. In the order from RNA1 to RNA6, these genomic RNAs contained ORFs coding for the RNA-dependent RNA polymerase (RdRp, p1 of 266 kDa), the glycoprotein precursor (GP, p2 of 74.5 kDa), the nucleocapsid (NC, p3 of 34.9 kDa), and the putative movement protein (MP, p4 of 40.7 kDa), while p5 (55 kDa) and p6 (27 kDa) had unknown functions. All RNA segments showed distant relationships to viruses of the genus Emaravirus, and in particular to pigeonpea sterility mosaic virus (PPSMV), with which they shared nucleotide sequence identity ranging from 48.5 % (RNA3) to 62.5 % (RNA1). In phylogenetic trees constructed from the sequences of the proteins encoded by RNA1, RNA2 and RNA3 (p1, p2 and p3), this new viral entity showed a consistent grouping with fig mosaic virus (FMV) and rose rosette virus (RRV), which formed a cluster of their own, clearly distinct from PPSMV-1. In experimental greenhouse trials, this novel virus was successfully transmitted to pigeonpea and French bean seedlings by the eriophyid mite Aceria cajani. Preliminary surveys conducted in the Hyderabad region (India) showed that the virus in question is widespread in pigeonpea plants affected by sterility mosaic disease (86.4 %) but is absent in symptomless plants. Based on molecular, biological and epidemiological features, this novel virus is the second emaravirus infecting pigeonpea, for which the provisional name pigeonpea sterility mosaic virus 2 (PPSMV-2) is proposed.