Characterization of Oxygen Transfer in Vertical Microbubble Columns for Aerobic Biotechnological Processes
Characterization of Oxygen Transfer in Vertical Microbubble Columns for Aerobic Biotechnological Processes
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DOI：
10.1002/bit.25243
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发表时间：
2014-09-01
影响因子：
3.8
通讯作者：
Krull, Rainer
中科院分区：
文献类型：
--
作者：
Peterat, Gena;Schmolke, Hannah;Krull, Rainer
本文介绍了微技术制造的微泡柱作为深层需氧培养筛选工具的适用性。在直立放置的微型装置的底部产生直径数百微米的气泡。上升的气泡引起液体循环，从而通过减少扩散距离并防止细胞沉降来增强混合。测试了两种不同尺寸的喷嘴（横截面为 21 x 40 μm(2) 和 53 x 40 μm(2)）。气体流速可调，并通过图像分析研究产生的气泡尺寸和含气量。该微型设备具有用于实时在线监测光密度和溶解氧的传感器元件。微型装置的主动通气可实现灵活的供氧，传质速率高达 0.14 s(-1)。配备较小喷嘴的微泡塔具有稍高的氧气传质速率和更好的脱气效果。为了验证微泡柱在好氧深层培养过程中的适用性，对模式生物酿酒酵母进行了批量培养，并对其比生长速率、摄氧速率和产量系数进行了研究。 (c) 2014 年 Wiley 期刊公司。
This paper presents the applicability of a microtechnologically fabricated microbubble column as a screening tool for submerged aerobic cultivation. Bubbles in the range of a few hundred micrometers in diameter were generated at the bottom of an upright-positioned microdevice. The rising bubbles induced the circulation of the liquid and thus enhanced mixing by reducing the diffusion distances and preventing cells from sedimentation. Two differently sized nozzles (21 x 40 mu m(2) and 53 x 40 mu m(2) in cross-section) were tested. The gas flow rates were adjustable, and the resulting bubble sizes and gas holdups were investigated by image analysis. The microdevice features sensor elements for the real-time online monitoring of optical density and dissolved oxygen. The active aeration of the microdevice allowed for a flexible oxygen supply with mass transfer rates of up to 0.14 s(-1). Slightly higher oxygen mass transfer rates and a better degassing were found for the microbubble column equipped with the smaller nozzle. To validate the applicability of the microbubble column for aerobic submerged cultivation processes, batch cultivations of the model organism Saccharomyces cerevisiae were performed, and the specific growth rate, oxygen uptake rate, and yield coefficient were investigated. (c) 2014 Wiley Periodicals, Inc.