Genetic characterization and geographic distribution of rabies virus isolates in Brazil: identification of two reservoirs, dogs and vampire bats.
Genetic characterization and geographic distribution of rabies virus isolates in Brazil: identification of two reservoirs, dogs and vampire bats.
复制标题
巴西狂犬病病毒分离株的遗传特征和地理分布：识别两个宿主：狗和吸血蝙蝠。
作者：
Mikako Ito;Y. T. Arai;T. Itou;T. Sakai;F. H. Ito;T. Takasaki;I. Kurane
我们分析了从巴西分离的50份狂犬病病毒样本，这些样本来自12只狗、11只猫、5只吸血蝙蝠、15头牛、2匹马、1头猪、1只羊和3个人，以调查狂犬病病毒的分子流行病学。我们通过PCR扩增产物的直接测序对核蛋白基因上的203个核苷酸进行了测序。所有分离株均属于基因1型，203个核苷酸的同源性至少为83.7%。根据核苷酸序列的同源性估计了主要储库。巴西狂犬病病毒分离株分为两个水库组：狗和吸血蝙蝠。所有犬源狂犬病病毒分离株的核苷酸同源性均大于99.0%。吸血蝙蝠相关的狂犬病病毒分离株显示核苷酸同源性大于96.6%，并可以进一步分为相应的病毒分离的地区亚组。这些数据表明，在巴西，流行的狂犬病变种至少属于两个不同的基因型簇，这两个簇在吸血蝙蝠和狗之间独立存在。
We analyzed 50 rabies virus samples isolated in Brazil from 12 dogs, 11 cats, 5 vampire bats, 15 cattle, 2 horses, 1 pig, 1 sheep, and 3 humans to investigate the molecular epidemiology of rabies viruses. We sequenced 203 nucleotides on the nucleoprotein gene by direct sequencing of the PCR-amplified products. All the isolates belonged to the genotype 1 and homology of the 203 nucleotides was at least 83.7% among isolates. The main reservoirs were estimated based on the homology of nucleotide sequences. Brazilian rabies virus isolates were clustered into two reservoir groups: dogs and vampire bats. All the dog-related rabies virus isolates showed nucleotide homology greater than 99.0%. Vampire bat-related rabies virus isolates showed nucleotide homology greater than 96.6% and could be further divided into subgroups corresponding to areas where viruses were isolated. These data suggest that circulating rabies variants belong to at least two different genotype clusters in Brazil and that these two clusters are maintained independently among vampire bats and dogs.