Study on the effect of Periplaneta americana on ulcerative colitis in rats induced by 2,4,6-trinitrobenzene sulfonic acid
Study on the effect of Periplaneta americana on ulcerative colitis in rats induced by 2,4,6-trinitrobenzene sulfonic acid
复制标题
美洲大蠊对2,4,6-三硝基苯磺酸所致大鼠溃疡性结肠炎的作用研究
DOI：
10.1177/2058739220942629
复制
发表时间：
2020
影响因子：
0.7
通讯作者：
Cheng-Gui Zhang
中科院分区：
文献类型：
--
作者：
Yi-Hao Che;Zhi-Bin Yang;Han-Chao Zhang;Xiu-Mei Wu;Min-Zhe Sun;Miao Tang;Peng-Chuan Wang;Fu-Neng Geng;Ping Wan;Heng Liu;Miao He;Yu Zhao;Cheng-Gui Zhang
溃疡性结肠炎（UC）是一种慢性肠道炎症性疾病，美洲大蠊是治疗UC的有效药物。研究美洲大蠊提取物Ento-A对2，4，6-三硝基苯磺酸（TNBS）诱导的大鼠UC的治疗作用，并探讨其作用机制。将SD大鼠随机分为正常对照组、TNBS组、柳氮磺胺吡啶（SASP）组、Ento-A低（50 mg/kg）、中（100 mg/kg）、高（200 mg/kg）剂量组。通过TNBS诱导大鼠UC模型。采用疾病活动指数（DAI）评价UC的严重程度。采用结肠黏膜损伤指数（CMDI）和组织病理学评分（HS）评价结肠的大体和显微损伤。采用酶联免疫吸附法（ELISA）检测血清白细胞介素4（IL-4）、白细胞介素17（IL-17）、肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）水平及结肠黏膜髓过氧化物酶（MPO）、转化生长因子-β1（TGF-β1）、表皮生长因子（EGF）含量。与正常对照组相比，TNBS治疗组DAI、CMDI、HS、IL-17、TNF-α、IFN-γ和MPO水平升高，IL-4、EGF和TGF-β1水平降低。然而，Ento-A给药组逆转了TNBS引起的DAI、CMDI、HS和细胞因子的变化。研究表明，美洲大蠊提取物Ento-A能有效减轻TNBS诱导的UC大鼠炎症反应，其机制可能与恢复Th 1/Th 2/Th 17/Treg细胞因子的平衡有关。
Ulcerative colitis (UC) is a chronic inflammatory disease of intestinal tract, and Periplaneta americana has been found to be effective in the treatment for UC. The purpose of the study was to investigate the therapeutic effect of Periplaneta americana extract Ento-A on UC in rats induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) and to explore its mechanism. The Sprague-Dawley (SD) rats were randomly divided into normal control group; TNBS-treated group; sulfasalazine (SASP) treated group; Ento-A low- (50 mg/kg), medium- (100 mg/kg), and high-dose (200 mg/kg) groups, respectively. The UC model of rats was induced via TNBS. Disease activity index (DAI) was used to evaluate the severity of UC in rats. The macroscopic and microscopic damages of colon were accessed by colon mucosa damage index (CMDI) and histopathological score (HS), respectively. The levels of interleukin-4 (IL-4), interleukin-17 (IL-17), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ) in serum and the contents of myeloperoxidase (MPO), transforming growth factor-β1 (TGF-β1), and epidermal growth factor (EGF) in colonic mucosa were measured by enzyme-linked immunosorbent assay (ELISA). Compared with the normal control group, the TNBS-treated group showed increase in DAI, CMDI, HS, IL-17, TNF-α, IFN-γ as well as MPO and decrease in the levels of IL-4, EGF, and TGF-β1. However, Ento-A-administrated groups reversed the changes in the DAI, CMDI, HS, and the cytokines caused by TNBS. The study indicates that Periplaneta americana extract Ento-A can effectively alleviate the inflammation in TNBS-induced UC of rats, and the mechanism of that may be related to restoring the balance of T helper 1 (Th1)/Th2/Th17/T regulatory (Treg) cytokines.