A quantitative observation and imaging of single tumor cell migration and deformation using a multi-gap microfluidic device representing the blood vessel
A quantitative observation and imaging of single tumor cell migration and deformation using a multi-gap microfluidic device representing the blood vessel
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DOI：
10.1016/j.mvr.2006.06.003
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发表时间：
2006-11-01
影响因子：
3.1
通讯作者：
Swaminathan, S.
中科院分区：
文献类型：
--
作者：
Chaw, K. C.;Manimaran, M.;Swaminathan, S.
开发了一种微流体装置，用于使用直接成像来量化单个肿瘤细胞的迁移和变形能力。它是用光刻法制造的，由聚二甲基硅氧烷制成。趋化性方法用于指导细胞运动，使用10 μ m的微间隙来限制迁移到单个细胞。每个细胞的迁移率被量化为随时间推移移动的距离的量度。实时记录细胞在生理流动条件下的变形情况，比较细胞的伸长指数和表面积的变化。使用三种人肿瘤细胞系即HepG 2、HeLa和MDA-MB-435 S来验证装置的操作和方法。它们的迁移速度为5至15 μ m/h，与其他科学报告一致。通过将微间隙宽度减小到3 μ m，发现细胞沿着微间隙行移动，但不能迁移穿过微间隙。随后通过间隙的细胞的变形进一步表明，它们的迁移能力可能由它们的变形能力和它们的膜上的变形应力决定。靶向癌细胞膜破裂的策略可能为转移提供治疗。作为一个有价值的工具，快速定量的单细胞的迁移能力，该装置应有助于药理学和药物筛选，调查的因素，调节细胞迁移和变形。(c)2006年爱思唯尔公司All rights reserved.
A microfluidic device was developed for quantifying the migratory and deformability capabilities of a single tumor cell using direct imaging. It was fabricated using photolithography and is made of polydimethysiloxane. Chemotaxis approach was used for directing cell movement, using 10 mu m microgaps to restrict the migration to a single cell. Each cell's migration rate is quantified as a measure of its distance traveled over time taken. Real-time recording of cell deformation under physiological flow was performed, and the elongation index and surface area change of the cells were compared. Three human tumor cell lines viz. HepG2, HeLa and MDA-MB-435S were used to verify the operation and methodology of the device. Their migration rates ranged from 5 to 15 mu m/h, consistent with other scientific reports. By reducing the microgap width to 3 mu m, it was found that the cells moved along the row of microgaps but were unable to migrate across the microgaps. Subsequent deformation of the cells through the gaps further showed that their migratory capability might be governed by their deformation ability and the deformation stress on their membranes. The strategy of targeting cancer cell membrane for rupture may provide a therapy for metastasis. Being a valuable tool for rapid quantification of a single cell's migratory capability, this device should be helpful for pharmacologic and drug screening, investigation of factors that regulate cell migration and deformation. (c) 2006 Elsevier Inc. All rights reserved.