Identification of a Novel Astrovirus (Astrovirus VA1) Associated with an Outbreak of Acute Gastroenteritis
Identification of a Novel Astrovirus (Astrovirus VA1) Associated with an Outbreak of Acute Gastroenteritis
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DOI：
10.1128/jvi.00998-09
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发表时间：
2009-10-15
影响因子：
5.4
通讯作者：
Tong, Suxiang
中科院分区：
文献类型：
--
作者：
Finkbeiner, Stacy R.;Li, Yan;Tong, Suxiang
大部分胃肠道疾病的病因尚不清楚。在这项研究中，使用随机桑格测序和焦磷酸测序方法分析了儿童保育中心不明原因胃肠炎暴发的粪便标本。鉴定出与已知星状病毒具有有限同一性的多个序列。序列组装、随后的逆转录 PCR (RT-PCR) 以及 cDNA 末端的快速扩增生成了 6,586 个核苷酸的完整基因组。系统发育分析表明，这种病毒被命名为星状病毒 VA1 (AstV-VA1)，与之前描述的所有星状病毒高度不同。根据 RT-PCR，本次疫情中多名患者的标本均呈 Ast-VA1 明确阳性。
The etiology of a large proportion of gastrointestinal illness is unknown. In this study, random Sanger sequencing and pyrosequencing approaches were used to analyze fecal specimens from a gastroenteritis outbreak of unknown etiology in a child care center. Multiple sequences with limited identity to known astroviruses were identified. Assembly of the sequences and subsequent reverse transcription-PCR (RT-PCR) and rapid amplification of cDNA ends generated a complete genome of 6,586 nucleotides. Phylogenetic analysis demonstrated that this virus, named astrovirus VA1 (AstV-VA1), is highly divergent from all previously described astroviruses. Based on RT-PCR, specimens from multiple patients in this outbreak were unequivocally positive for Ast-VA1.