THE INTERACTION OF AMINOGROUPS WITH PYRROLOQUINOLINE QUINONE AS DETECTED BY THE REDUCTION OF NITROBLUE TETRAZOLIUM
THE INTERACTION OF AMINOGROUPS WITH PYRROLOQUINOLINE QUINONE AS DETECTED BY THE REDUCTION OF NITROBLUE TETRAZOLIUM
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DOI：
10.1016/s0006-291x(88)81230-0
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发表时间：
1988-05-31
影响因子：
3.1
通讯作者：
GALLOP, PM
中科院分区：
文献类型：
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作者：
FLUCKIGER, R;WOODTLI, T;GALLOP, PM
通过测定加合物对硝基蓝四唑（NBT）的还原能力，研究了吡咯喹啉醌（PQQ）与氨基的相互作用。天然氨基酸中只有甘氨酸、鸟氨酸和赖氨酸与PQQ有强烈的相互作用。除赖氨酸外，氨、伯胺或仲胺均能提高其他活性较弱的氨基酸的还原活性。相反，二价阳离子抑制nbt还原活性的发展。PQQ在血清素和白蛋白存在时也表现出nbt反应性。提出了一个反应方案来解释这些发现。这表明血浆中nbt的还原活性不是由血浆蛋白糖基化引起的，而是由血浆中固有的PQQ加合物引起的。这种nbt还原活性约对应于10 .mu。g PQQ/ml血浆。
The interaction of pyrroloquinoline quinone (PQQ) with amino groups was followed by measuring the capacity of adducts to reduce nitroblue tetrazolium (NBT). Of the natural amino acids only glycine, ornithine, and lysine interacted strongly with PQQ. The reducing activity of other less reactive amino acids, but not of lysine, was increased by ammonia, primary or secondary amines. Divalent cations, in contrast inhibited development of NBT-reducing activity. PQQ also developed NBT-reactivity in the presence of serotonin and albumin. A reaction scheme is proposed which explains these findings. It is suggested that the NBT-reducing activity of plasma which is not caused by glycation of plasma proteins, arise from PQQ adducts inherent to plasma. This NBT-reducing activity corresponds to approximately 10 .mu.g PQQ/ml plasma.