Characterization of microminipig as a laboratory animal for pharmacological study by analyzing bepridil-induced cardiovascular responses
Characterization of microminipig as a laboratory animal for pharmacological study by analyzing bepridil-induced cardiovascular responses
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通过分析贝普地尔诱导的心血管反应来表征微型猪作为药理学研究的实验动物
DOI：
10.1016/j.jphs.2020.01.010
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发表时间：
2020
影响因子：
3.5
通讯作者：
Sugiyama Atsushi
中科院分区：
文献类型：
--
作者：
Nunoi Yoshio;Hagiwara-Nagasawa Mihoko;Kambayashi Ryuichi;Goto Ai;Chiba Koki;Wada Takeshi;Izumi-Nakaseko Hiroko;Matsumoto Akio;Watanabe Yoshinori;Sugiyama Atsushi
由于微型猪成为各种心脏电药理学应用的有吸引力的模型，这可能符合3Rs的考虑。我们通过分析多离子通道抑制剂苄普地尔如何影响它们的原位心脏来表征微型猪，并与狗进行比较。在氟烷麻醉下，静脉注射苄普地尔0.3和3.0mg/kg，10 min内给药，结果表明，小型猪对苄普地尔抑制ICa T的敏感性较低，而对INa、IKs和IKs的抑制反应较强。这一信息将有助于预测药物对具有与微型猪相似的电生理特征的重塑心脏患者的心血管作用。
Sincemicrominipigis becoming attractive model for various cardiac electropharmacological applications, which may meet consideration of 3Rs. We characterizedmicrominipigsby analyzing how multi-ionic channel inhibitor bepridil may affect their in situ hearts in comparison with dogs. Bepridil in doses of 0.3 and 3.0 mg/kg were intravenously administered over 10 min under halothane anesthesia (n = 4).Microminipigsmay be less sensitive for ICaTinhibition of bepridil, whereas they are more responsive to INa, IKrand IKssuppression than dogs. This information would help predict cardiovascular effects of a drug in patients with the remodeled hearts having similar electrophysiological profile tomicrominipigs.