Quantification of mitochondrial DNA copy number: Pre-analytical factors
Quantification of mitochondrial DNA copy number: Pre-analytical factors
复制标题
DOI：
10.1016/j.mito.2009.02.006
复制
发表时间：
2009-07-01
期刊：
影响因子：
4.4
通讯作者：
Garcia-Arumi, Elena
中科院分区：
文献类型：
--
作者：
Andreu, Antonio L.;Martinez, Ramiro;Garcia-Arumi, Elena
线粒体DNA（mtDNA）含量对于理解许多细胞过程是重要的。从样品采集到DNA提取的几个预分析因素会影响mtDNA拷贝数的测量。在本研究中，全血样本比血沉棕黄层样本产生更高的mtDNA拷贝数。mtDNA含量受所使用的细胞分离方法以及采血和细胞分离之间的时间的影响。因此，必须使用相同类型的样品建立参考值。至于DNA分离和纯化方法，人工酚法可能会随机产生假高值。QIAamp DNA Mini Kit提供了最高可重复性的mtDNA/nDNA产量。(C)2009 Elsevier B. V.和线粒体研究学会。All rights reserved.
Mitochondrial DNA (mtDNA) content is important for understanding many cellular processes. Several pre-analytical factors, from sample collection to DNA extraction can affect measurement of mtDNA copy number. In the present study, whole blood samples yielded a higher mtDNA copy number than buffy coat samples. mtDNA content is affected by the cell separation method used and the time between blood withdrawal and cell separation. Thus, reference values must be established with the same type of sample. As to the DNA isolation and purification method, the manual phenol method can give randomly false high values. The QIAamp DNA Mini Kit provided the most highly reproducible mtDNA/nDNA yield. (C) 2009 Elsevier B.V. and Mitochondria Research Society. All rights reserved.