BACTERIAL-CELL SURFACE HYDROPHOBICITY PROPERTIES IN THE MEDIATION OF INVITRO ADHESION BY THE RABBIT ENTERIC PATHOGEN ESCHERICHIA-COLI STRAIN RDEC-1
BACTERIAL-CELL SURFACE HYDROPHOBICITY PROPERTIES IN THE MEDIATION OF INVITRO ADHESION BY THE RABBIT ENTERIC PATHOGEN ESCHERICHIA-COLI STRAIN RDEC-1
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DOI：
10.1172/jci114336
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发表时间：
1989-11-01
影响因子：
15.9
通讯作者：
SHERMAN, PM
中科院分区：
文献类型：
--
作者：
DRUMM, B;NEUMANN, AW;SHERMAN, PM
疏水性在肠道病原体附着到胃肠粘膜中的作用存在争议。大肠杆菌RDEC-1与兔肠道的体外结合依赖于菌毛的表达。我们在改变生物体的疏水性、附着底物的疏水性或悬浮液的表面张力后，检测了带毛的RDEC-1的体外黏附。用四种互补方法测定RDEC-1的疏水性。在每个实验中，与抑制菌毛表达的微生物和不能表达甘露糖抗性菌毛的突变体相比，带毛的RDEC-1表现出相对更多的疏水性。当纤毛RDEC-1用四甲基脲破坏疏水键时，表面疏水性降低。同时，细菌对兔回肠微绒毛膜、粘液和粘蛋白的粘附率降低。与非纤毛细菌和相对亲水性表面的粘附性RDEC-1相比，纤毛生物与疏水表面的结合显著更高。丙醇的加入降低了悬浮液的表面张力，使纤毛RDEC-1与聚苯乙烯的粘附性降低了80%。相反，在降低悬浮液的表面张力后，纤毛生物对亲水性表面的粘附性增加了12倍。我们得出结论，疏水特性在介导该大肠杆菌肠道病原体的体外黏附中起到了作用。
The role of hydrophobicity in the attachment of enteropathogens to gastrointestinal mucosa is controversial. In vitro binding of Escherichia coli RDEC-1 to rabbit intestine is dependent on the expression of pili. We examined in vitro adherence of piliated RDEC-1 after altering either the hydrophobicity of the organisms, the hydrophobicity of the substrate for attachment, or the surface tension of the suspending liquid. Hydrophobicity of RDEC-1 was determined using four complementary methods. In each assay piliated RDEC-1 demonstrated relatively more hydrophobic properties compared with both organisms grown to suppress pilus expression and a mutant that cannot express mannose-resistant pili. When piliated RDEC-1 were pretreated with tetramethyl urea to disrupt hydrophobic bonds surface hydrophobicity decreased. Concurrently, bacterial adherence to rabbit ileal microvillus membranes, mucus and mucin was reduced. Binding of piliated organisms to hydrophobic surfaces was significantly higher compared to both nonpiliated bacteria and the adherence of piliated RDEC-1 to relatively hydrophilic surfaces. Addition of propanol reduced the surface tension of the suspending liquid, and decreased adhesion of piliated RDEC-1 to polystyrene by 80%. Conversely, adherence of piliated organisms to a hydrophilic surface increased 12-fold after lowering the surface tension of the suspending liquid. We conclude that hydrophobic properties have a role in mediating in vitro adherence of this E. coli enteric pathogen.