Cloning and characterization of a novel cardiac-specific kinase that interacts specifically with cardiac troponin I
Cloning and characterization of a novel cardiac-specific kinase that interacts specifically with cardiac troponin I
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DOI：
10.1007/s00109-003-0427-x
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发表时间：
2003-05-01
影响因子：
4.7
通讯作者：
Ding, JF
中科院分区：
文献类型：
--
作者：
Zhao, Y;Meng, XM;Ding, JF
心脏限制性基因在心血管系统中起着重要作用。为了鉴定这些新基因，我们基于生物信息学分析鉴定了位于1p31.1的新的心脏特异性激酶基因TNNI 3 K。序列分析表明，TNNI 3 K是一个遥远的家族成员的整合素连接激酶。北方印迹和76-组织阵列分析表明，TNNI 3 K在心脏中高度表达，但在其他组织中检测不到。免疫组织化学分析主要定位在心肌细胞核中的TNNI 3 K。体外激酶试验表明TNNI 3 K是一种功能性激酶。酵母双杂交系统显示TNNI 3 K可以直接与心肌肌钙蛋白1相互作用，体内共免疫沉淀进一步证实了这一结果。提示TNNI 3 K是一种心脏特异性激酶，在心脏系统中发挥重要作用。
Cardiac-restricted genes play important roles in cardiovascular system. In an effort to identify such novel genes we identified a novel cardiac-specific kinase gene TNNI3K localized on 1p31.1 based on bioinformatics analyses. Sequence analysis suggested that TNNI3K is a distant family member of integrin-linked kinase. Northern blot and 76-tissue array analyses showed that TNNI3K is highly expressed in heart, but is undetectable in other tissues. Immunohistochemical analysis predominantly localized TNNI3K in the nucleus of cardiac myocytes. In vitro kinase assay showed that TNNI3K is a functional kinase. The yeast two-hybrid system showed that TNNI3K could directly interact with cardiac troponin 1, results that were further confirmed by coimmuno-precipitation in vivo. Our data suggest that TNNI3K is a cardiac-specific kinase and play important roles in cardiac system.