REGIONAL DIFFERENCES OF PROTEINS IN ISOLATED CELLS OF EARLY EMBRYOS OF XENOPUS-LAEVIS
REGIONAL DIFFERENCES OF PROTEINS IN ISOLATED CELLS OF EARLY EMBRYOS OF XENOPUS-LAEVIS
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DOI：
10.1016/0045-6039(87)90447-7
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发表时间：
1987-06-01
期刊：
影响因子：
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通讯作者：
KIHARA, HK
中科院分区：
文献类型：
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作者：
MIYATA, S;KAGEURA, H;KIHARA, HK
采用双向凝胶电泳技术研究了非洲爪蟾早期胚胎蛋白质的区域差异。从16-和32-细胞胚胎中分离出背腹轴上的成对卵裂球。在32细胞胚胎中检测到一些背腹差异。在腹侧边缘区的植物细胞中明显可检测到的蛋白质在背侧边缘区的植物细胞中仅微弱地检测到或检测不到，并且在背侧卵裂球中检测到区域特异性斑点。
Regional differences of proteins were studied by two-dimensional gel electrophoresis in early embryos of Xenopus laevis. Pairs of blastomeres on the dorso-ventral axis were isolated from 16- and 32-cell embryos. Some dorso-ventral differences have been detected at 32-cell embryos. The proteins which were clearly detectable in the vegetal cells of the ventral marginal zone were only faintly detectable or undetectable in those of the dorsal marginal zone, and a regionally specific spot was detected in dorsal blastomeres.